HPLC法同时测定麦味地黄胶囊中马钱苷、芍药苷和丹皮酚  

Simultaneous determination of loganin,paeoniflorin and paeonol in Maiwei Dihuang Capsules by HPLC

作　　者：冯发青[1] 王恩源 伍庆[1] 龙风荣 柳立伟[1] 洪江[3] 

机构地区：[1]贵州师范大学,贵州贵阳550001 [2]贵州弘康药业有限公司,贵州贵阳550001 [3]贵州科学院,贵州贵阳550001

出　　处：《中成药》2012年第12期2339-2342,共4页Chinese Traditional Patent Medicine

基　　金：国家科技支撑计划项目(2009BAI74B01-03);国家科技支撑计划项目(2009BAI74B02-01);国家"863"计划项目(2009AA043201)

摘　　要：目的建立同时测定麦味地黄胶囊(山茱萸、牡丹皮等)中马钱苷、芍药苷和丹皮酚的RP-HPLC方法。方法采用Diamonsil C18(4.6 mm×250 mm,5μm)色谱柱;流动相为乙腈-0.1%磷酸水溶液,梯度洗脱;体积流量1.0mL/min;检测波长235 nm;柱温30℃。结果马钱苷、芍药苷和丹皮酚分别在0.209～1.253μg(r=0.999 8)、0.182～1.089μg(r=0.999 7)和0.412～2.470μg(r=0.999 9)范围内呈良好的线性关系,平均回收率分别为98.22%(RSD为1.14%)、97.88%(RSD为0.90%)和99.10%(RSD为1.37%)。结论该法操作简便,测定结果准确,可用于控制麦味地黄胶囊成品质量。AIM To propose RP-HPLC method for simultaneously determining loganin,paeoniflorin and paeonol in Maiwei Dihuang Capsules（Corni Fructus,Moutan Cortex,etc.）.MEIHODS RP-HPLC analyses were carried out by using a Diamonsil C18（250 mm × 4.6 mm,5 μm）.The mobile phase was composed of acetonitrile-0.1% phosphric acid solution with the gradient elution.The flow rate was 1.0 mL/min and the detection wavelength was set at 235 nm.The column temperature was 30 ℃.RESULTS The calibration curves of loganin,paeoniflorin and paeonol were in good linearity over the ranges of 0.209-1.253 μg（r=0.999 8）,0.182-1.089 μg（r=0.999 7）,0.412~2.470 μg（r=0.999 9）,respectively and the average recoveries were 98.22%（RSD of 1.14%）,97.88%（RSD of 0.90%） and 99.10%（RSD of 1.37%）,respectively.CONCLUSION The method is simple,feasible and reproducible,and can be used for the quality control of Maiwei Dihuang Capsules.

关 键 词：麦味地黄胶囊 马钱苷 芍药苷 丹皮酚 HPLC 

分 类 号：R927.2[医药卫生—药学]