灵芝三萜组分GLE的体内外抗肿瘤作用  

Anti-tumor effect of triterpene GLE from Ganoderma Lucudum in vivo and in vitro

作　　者：刘锋[1,2] 李鹏[3,2] 黄秀旺[2] 许建华[1,2] 

机构地区：[1]福建医科大学药学院药理学系,福州350004 [2]福建医科大学药学院临床药理研究所,福建省高校天然药物药理学重点实验室,福州350004 [3]福建医科大学药学院天然药物学系,福州350004

出　　处：《中国新药杂志》2012年第23期2790-2793,共4页Chinese Journal of New Drugs

基　　金：福建省教育厅科技计划项目(JA11107);2011年福建省高校产学合作科技重大项目(2011Y4003)

摘　　要：目的:评价灵芝三萜组分GLE的体内外抗肿瘤作用。方法:建立小鼠S180实体瘤和腹水瘤两种模型,观察GLE对实体瘤抑瘤率和腹水瘤生命延长率的影响;采用MTT法检测GLE对人鼻咽癌细胞CNE1和CNE2的体外抗肿瘤活性。结果:3个剂量(125,250,500 mg.kg-1)GLE对S180实体瘤小鼠均具有一定的抑制作用(P<0.05),500 mg.kg-1组的瘤重抑制百分率达51.4%;对S180腹水瘤小鼠具有明显的延长存活期作用,500 mg.kg-1组的生命延长率为52.0%(P<0.05);体外对人鼻咽癌低分化细胞CNE2有一定的抑制作用。结论:GLE对小鼠肉瘤S180及人鼻咽癌低分化细胞CNE2的生长有一定的抑制作用。Objective： To evaluate the anti-tumor effect of triterpene GLE from Ganoderma Lucidum. Methods： The in vivo effect of GLE on solid tumor growth and the life span were determined in mice with S180- transplanted tumor. The in vitro cytotoxicity of GLE was evaluated by MTT reduction assay in CNE1 and CNE2 cells. Results： GLE （125,250,500 mg.kg-1） inhibited S180-transplanted tumor growth, and the inhibitive per- centage of carcinoma weight was 51.4% in 500 rag-kg-1 group. GLE prolonged the life span of mice with S180 tumor, and the average increase was 52.0% in 500 mg-kg-1 group （P 〈 0.05）. GLE had an inhibitory effect in CNE2 cancer cells in vitro. Conclusion： GLE can inhibit growth of S180 tumor in mice and CNE2 cells in vitro.

关 键 词：灵芝 抗肿瘤 S180 小鼠 体内外 

分 类 号：R979.1[医药卫生—药品]