胭脂花的组织培养与植株再生  

Tissue culture and plant regeneration of Primula maximowiczii

作　　者：宋韵霏[1] 王俊丽[1] 毕凯丽[1] 张璐[1] 李建飞[1] 

机构地区：[1]中央民族大学生命与环境科学学院,北京100081

出　　处：《河北大学学报（自然科学版）》2012年第6期630-634,共5页Journal of Hebei University(Natural Science Edition)

基　　金："973"计划资助项目(2009CB522300);中央高校基本科研业务费专项资金资助项目(0910KYZY46);国家民委资助项目(09ZY09);985工程资助项目(MUC985);高等学校学科创新引智计划资助项目(B08044)

摘　　要：为了有效保护和利用野生胭脂花资源,以胭脂花(Primula maximowiczii)叶片、叶柄作为外植体进行组织培养及植株再生研究,建立了胭脂花的离体无性繁殖体系.结果表明,叶柄不定芽诱导率较高.经实验筛选出最适不定芽诱导培养基为MS+2,4-D 1.0mg/L+TDZ 2.0mg/L,最佳不定芽增殖培养基为MS+2,4-D 4.0mg/L+6-BA 2.0mg/L,幼苗移栽成活率可达60%以上.A propagation system of Primula maximowiczii in vitro was established by using leaf ex- plants and petiole explants. The results showed that petiole explants has a higher adventitious shoot induction rate. The optimal medium for inducing adventitious shoots from petiole explants was MS＋2,4-D 1.0 mg/L＋ TDZ 2. 0 mg/L and the best medium for subculture was MS＋2,4-D 4. 0 mg/Lq- 6-BA 2.0 mg/L. The survival rate for regenerated plants was about 60%.

关 键 词：胭脂花 组织培养 植株再生 

分 类 号：Q945[生物学—植物学]