CD4^＋CD25^＋CD127-调节性T细胞在Graves病中的变化及临床意义  

Changes and clinical significance of CD4^＋CD25^＋CD127^- regulatory T cells in Graves disease

作　　者：邹劲涛[1] 余佩玲[2] 董经纬[1] 廖启洪[3] 刘东亮[1] 曾鸿毅[1] 

机构地区：[1]柳州市人民医院核医学科,545006 [2]柳州市人民医院医疗保健部,545006 [3]柳州市人民医院检验科,545006

出　　处：《国际放射医学核医学杂志》2012年第6期362-365,共4页International Journal of Radiation Medicine and Nuclear Medicine

基　　金：广西卫生厅立项科研项目（Z2009315）;柳州市科技局科研项目（2010030715）

摘　　要：目的通过观察Graves病患者外周血中CD4^＋D25℃D127^-调节性T细胞（Treg）数量的变化，探讨Graves病发生的免疫学机制。方法采用流式细胞术检测90例Graves病患者和50名正常人外周血中CD4^＋CD25^＋CD127^-Freg的比例，同时测定甲状腺功能指标及甲状腺自身抗体水平。采用t检验进行组间比较，采用线性相关分析法分析CD4^＋CD25^＋CD127^-Treg与甲状腺功能指标的关系。结果90例Graves病患者与50名正常人外周血中CD4^＋CD25^＋CD127^＋ Treg的比例分别为1．39％±1．09％和4．59％±1．14％，两者间差异有统计学意义（t=16．4，P〈0．01）。CD4^＋CD25^＋CD127^-Treg数量与促甲状腺激素受体抗体水平呈负相关（r=-0．24，P〈0．05），与总三碘甲状腺原氨酸、总甲状腺素、游离三碘甲状腺原氨酸、游离甲状腺素、甲状腺球蛋白抗体、甲状腺微粒体抗体水平呈负相关（r-0．62、-0．65、-0．56、-0．71、-0．50、-0．15，P均〈0．01）。结论Graves病患者CD4^＋CD25^＋CD127Freg数量减少，且CD4^＋CD25^＋CD127^-Treg数量与甲状腺功能指标及甲状腺自身抗体水平关系密切，提示CD4^＋CD25^＋CD127^- Freg数量减少可能与Graves病的发病有关。CD4^＋CD25^＋CD127^-可能是人CD4＋T细胞中Treg的特异性标志物。Objective To investigate the mechanism of Graves disease by observing the changes of CD4^＋CD25＋CD127^- regulatory T cells （Treg） population in the patients. Methods Flow cytometry was used to detect the proportion of CD4^＋CD25^＋CD127^- Treg of CD4^＋ T cells in 90 Graves disease patients （Graves disease group ） and 50 heahhy adults （control group ）. Thyroid function and autoantibody levels were deter- mined simultaneously. The t test was adopted for comparison between groups. The relationship between CD4^＋CD25^＋CD 127^- Treg and thyroid function was analyzed by linear correlation analysis. Results The percentages of CD4^＋CD25＋CD127^-Freg in Graves disease group and control group were 1.39%±1.09% and 4.59%±1.14% separately. There was significant difference between the two groups （t=16.4, P〈0.01 ）. There were negative correlation between CD4^＋CD25＋CD127^- Treg percentages and total triiodothyronine, total thyroxine, free triiodothyronine, free thyroxine and thyrotropin receptor antibody, thyroglobulin antibody, thyroid microsomal antibody （r=-0.62, -0.65, -0.56, -0.71, -0.50, -0.15, all P〈0.01）. Conclusions The reduction of CD4^＋CD25^＋CD127^-Freg percentages in Graves disease group and close relations of CD4^＋CD25＋CD127^-Freg with thyroid function and thyroid autoantibody levels suggest that CD4^＋CD25＋CD127^-Freg decrease in the num- ber may be associated with the onset of Graves disease. CD4^＋CD25^＋CD127^- may be the specific marker of Treg.

关 键 词：格雷夫斯病 T淋巴细胞 调节性 CD4 抗原 CD25 CD127 

分 类 号：R581.1[医药卫生—内分泌]