针刺对光老大鼠皮肤组织中Bcl-2、Bax表达的影响及意义  

The Effect and Significance of Acupuncture on the Expression of Bcl-2 and Bax of Photoaing Rats

作　　者：陆佳宁[1] 张小卿[1] 田国伟[1] 陶冶[1] 吴景东[1] 

机构地区：[1]辽宁中医药大学,辽宁沈阳110847

出　　处：《中华中医药学刊》2012年第12期2695-2696,I0023,共3页Chinese Archives of Traditional Chinese Medicine

基　　金：辽宁省教育厅科学研究项目(2008T122)

摘　　要：目的:观察Bcl-2、Bax蛋白在光老化大鼠皮肤组织中的表达状况,探讨针刺抗大鼠皮肤光老化的作用机制。方法:将大鼠随机分成正常组、模型组、针刺组、阳性对照组,除正常组外,其余各组模拟日光中UV(UVA+UVB)照射,造成皮肤光老化模型,每次造模前,针刺组给予电针刺激,阳性对照组采用VE涂抹造模部位,15周后取皮肤组织采用免疫组织化学方法检测Bcl-2、Bax蛋白的表达水平,利用计算机图像分析技术测量单位面积各组大鼠皮肤组织Bcl-2、Bax蛋白表达的灰度平均值作为其表达情况。结果:与正常组比较,模型组大鼠Bcl-2蛋白表达明显降低(P<0.05),Bax蛋白表达明显升高(P<0.05)。针刺组和阳性对照组与模型组比较,Bcl-2蛋白表达提高(P<0.05),Bax蛋白表达明显降低(P<0.05)。结论:Bcl-2、Bax蛋白参与了皮肤光老化进程,针刺能够抑制皮肤光老化皮肤细胞凋亡,上调Bcl-2蛋白表达、下调Bax蛋白表达。Objective：To study the expression of acupuncture on the the activity of Bcl -2B -cell lymphoma/leukae- mia- 2gene （Bcl -2） and Bcl- 2 Assaciated X protein （Bax） on cutaneous tissue of photoaging rats and to investigate the the mechanism of acupuncture on photoaging rats. Methods ： The rats were divided into 4 groups, normal group, model group, acupuncture group, positive group, except for the normal control group, the rats of other groups all accepted the ar- tificial UV radiation（ UVA ＋ UVB ）, the rats of acupuncture group accepted Electro acupuncture, Vitamin E cream was used on rats back in positive control group, fiften weeks later, compared the average gray level of Bel - 2, Bax on cutane- ous tissue in unit area of the rats in each groups by computer image analysis technique. Resuhs ： comparing with the normal control group, the expression of Bcl -2 in model group was significantly decreased（ P 〈 0.05 ）, the expression of Bcl -2 in model was significantly increased （P 〈 O. 05 ）. Comparing with the positive group and model group, the expression of Bcl - 2 in aupuncture group was significantly increased （ P 〈 0.05 ）, the expression of Bax in acupuncture was significantly decreased（ P 〈 O. 05） . Conclusion：Bcl- 2 and Bax involved in the process of photoaging, acupuncture could inhibit the apoptosis of photoaing skin by up regulating the expression of Bcl -2 and down regulating the expression of Bax.

关 键 词：针刺 光老化 Bcl-2 BAX 细胞凋亡 免疫组织化学 

分 类 号：R245.31[医药卫生—针灸推拿学]