郁金提取物对福尔马林致痛小鼠行为学及大脑皮层NF-κB蛋白表达的影响  

Study of Radix Curcuma Extracts on Formalin-induced Paw Licking Tests and NF-κB Expression in Cerebral Cortex of Mice

作　　者：许俊杰 龚敏操[2] 陈眉[2] 

机构地区：[1]嘉兴市第一医院,浙江嘉兴314000 [2]浙江省中医院,浙江杭州310006

出　　处：《中华中医药学刊》2012年第12期2707-2709,I0026,I0027,共5页Chinese Archives of Traditional Chinese Medicine

基　　金：浙江省中医药科研基金资助项目(2009CA012)

摘　　要：目的:研究郁金提取物对福尔马林引起的炎性疼痛小鼠的镇痛作用。方法:采用清醒小鼠单侧足底皮下注射5%福尔马林20μL,观察各组小鼠Ⅰ、Ⅱ相的累计舔足时间;行为学观察后取小鼠大脑皮皮层组织,采用免疫组化法测定各组小鼠大脑皮层SI区NF-κB蛋白的表达。结果:福尔马林模型能使小鼠出现明显的两相伤害性行为反应,郁金提取物能抑制小鼠福尔马林实验的Ⅱ相反应(P<0.01),能降低小鼠大脑皮层SI区NF-κB蛋白的表达(P<0.05),其结果具有一定的剂量相关性。结论:郁金提取物对福尔马林引起的炎性疼痛具有良好的镇痛作用,其机制可能于抑制NF-κB参与的炎症反应有关。Objective ： To evaluate the analgesic effects of Radix Curcuma extracts on inflammatory pain in mice. Meth ods ： After injection of 5% formalin 20μL, the pain threshold and accumulative licking feet times of each phase were deter mined and compared among all groups of mice, respectively. The expressions of NF - KB in brain cortex of all groups were measured through immunohistochemistry assay. Results ： Injection of formalin can elicit acute two - phase noxious reac tion. Radix Curcuma extracts significantly abrogated the formalin -induced second phase painful reaction in mice model compared with that of control（P 〈 0. O1 ）, and Radix Curcuma extracts showed remarkable dose- dependent down- regu lation of NF - KB protein expression in cerebral cortex when compared with that of model control（P 〈 O. 05）. Conclusion： Radix Curcuma extracts exerted significant antinociceptive activity in experimental animals. The mechanism may be relat- ed to reducing the expression of inflammatory cytokines involving inflammatory reaction.

关 键 词：郁金 Β-榄香烯 NF-ΚB 镇痛 小鼠 

分 类 号：R285.5[医药卫生—中药学]