不同产地及不同采收期北柴胡中柴胡总皂苷的含量测定  

Content Determination of Saikosaponins in Bupluerum Chinense from Different Origins and Different Harvest Time

作　　者：卫冰[1] 李晓坤[1] 杨云[1] 王启帅[1] 刘富岗[1] 

机构地区：[1]河南中医学院药学院,河南郑州450008

出　　处：《辽宁中医杂志》2012年第12期2464-2466,共3页Liaoning Journal of Traditional Chinese Medicine

基　　金：国家"十一五"科技支撑计划资助项目(2006BAIO06A10);河南省科技成果转化(092201310001)

摘　　要：目的:测定不同产地及不同采收期北柴胡中柴胡总皂苷的含量。方法:以柴胡皂苷a为对照品,采用紫外可见分光光度法,于535nm处测定不同产地及同一产地不同采收期北柴胡样品中柴胡总皂苷的含量。结果:柴胡总皂苷在0.0306～0.3062 mg具有良好的线性关系(r=0.9996),加样回收率为101.44%,RSD为3.29%,不同产地北柴胡中柴胡总皂苷含量为0.98%～2.05%;生长年限为19个月(9月28号采集)的河南嵩县北柴胡柴胡总皂苷含量达到最大富集值,为1.78%。结论:不同产地及同一产地不同采收期的北柴胡药材中的总皂苷含量存在明显差异,河南嵩县北柴胡的最佳采收期为生长周期第二年的9月28号。Objective：To determine the contents of saikosaponins in Bupluerum chinense from different origins and different harvest time. Methods：The contents of saikosaponins were measured with UV-vis spectrophotometry at 535nm and saikosaponin-a was used as the standard substance. Results ：The calibration curve had good linear relationship in range of 0. 0306 -0. 3062mg（ r = 0.9996）. The average recovery was 101.44% and the RSD was 3.29%. The contents of saikosaponins in Bupluerum chinense from different origins were 0.98% -2.05 %. The Bnpluerum Chinense from Songxian（ Henan province）got the highest contents of saikosaponins when the growth period was 19 months（ collection period was on September 28th） and the contents were 1.78%. Conclusion ： The contents of saikosaponins from different origins and different harvest time （ same origin） were obviously different and the best harvest time for Bupluerum chinen＇e from Songxian region was on September 28th in the second year of growth cycle.

关 键 词：北柴胡 柴胡皂苷A 柴胡总皂苷 紫外可见分光光度法 

分 类 号：R927.2[医药卫生—药学]