毛细管区带电泳法分离检测神经性毒剂降解产物  

Determination of Degradation Products of Six Nerve Agent by Capillary Zone Electrophoresis

作　　者：刘勤[1] 周永新[1] 汤立合 刘荫棠[1] 

机构地区：[1]北京毒物药物研究所,北京100850

出　　处：《分析化学》2000年第7期810-814,共5页Chinese Journal of Analytical Chemistry

摘　　要：用毛细管区带电泳间接紫外检测法分离测定６种甲基膦酸单酯。以苯基膦酸为紫外添加剂，检测波长为 ２１０ ｎｍ。优化后缓冲溶液组成为：２００ ｍｍｏｌ／Ｌ硼酸－５ ｍｍｏｌ／Ｌ苯基膦酸－０．１０ ｍｍｏｌ／Ｌ ＤＤＡＢ－０．２０％Ｔｒｉｔｏｎ Ｘ－１００（ｐＨ ３．５５）。毛细管规格为７０ ｃｍ ｘ ７５ μｍ ｉ．ｄ。以ＫＨ２ｎＰＯ４为内标定量，在１～３０ｍｇ／Ｌ浓度范围内６种甲基膦酸单酯标准回归曲线线性关系良好（ｒ＞０．９９８９），检测限为０．２５～０．５ｍｇ／Ｌ。总分析时间在１４ ｍｉｎ以内。Capillary zone electrophoresis with indirect UV detection was used for the determination of sir methylphoophonic acid monoesters. Phenylphosphonic acid was used as a background absorption agent. The optimized electrolyte contains 200 mmol/L boric acid, 5mmol/L phenylphosphonic acid, 0. l0mmol/L didodecyldimethylammonium bromide (DDAB ) and 0. 20 % Triton X- 100 (pH 3. 55 ). The capillary used was70 cm x 75 μp(i. d.) and detection was carried out at 210 um. KH2PO4 was used as internal standard. The calibration curve showed a good linearity for six methylphosphonic acid monoesters in the concentration range of 1 ~ 30 mg/L (r > 0.9989) and the detection limits were 0.25 ~ 0. 5 mg/L. The total the for separation and determination was within 14 min.

关 键 词：毛细管区带电泳 神经性毒剂 降解产物 分离 检测 

分 类 号：O658.9[理学—分析化学]