高效液相色谱法测定生物组织中的组胺  

Determination of Histamine in Biological Sample by High Performance Liquid Chromatography with Fluorescence Detection After Post Column-Derivatization

作　　者：黄新华[1] 陈本美[2] 梁绍先[2] 邓富良[2] 

机构地区：[1]湖南医科大学设备处,长沙410078 [2]湖南医科大学分析测试中心,长沙410078

出　　处：《分析化学》2000年第7期865-867,共3页Chinese Journal of Analytical Chemistry

摘　　要：通过比较各种分析柱的分离效果，建立了用离子交换柱分离，邻苯二甲醛（ＯＰＡ）柱 后衍生荧光检测分析生物组织中组胺的液相色谱方法，并对流动相的离子强度和ｐＨ进行了 优化，确定以４０ｍｍｏｌ／Ｌ　ｐＨ５．５０的柠檬酸钠缓冲液作为流动相为最佳条件。该方法的检测限 为５０ ｎｍｏｌ／Ｌ，回收率在 ９２％以上，线性范围为 ５０ ｎｍｏｌ／Ｌ～ ５００ μｍｏｌ／Ｌ。分析时间缩短为 １５ ｍｉｎ。A rapid and simple method for the determination of histamine in biological sample by postcolumn derivatization liquid chromatography with fluorescence detection was described. A SCX weakly acidic cation exchange column was used as an analytical column. Histamine was eluted at 13. 2 min by 40 mmol/L of trisodium citrate (pH 5 .50) at flow rate of 1. 0 mL/min. The recoveries of histamine from 1 .0 μmol/L to 100 were above 92%. The detection limit was 50nmol/L and the linear of the determination was 50 nmol/L ~ 500 μmol/L histamine.

关 键 词：组胺 高效液相色谱法 生物样品 

分 类 号：O657.7[理学—分析化学]