基于微腔型电极的半乳糖生物传感器  

A Chamber-type Microchip-based Microchip-based Biosensor

作　　者：贾能勤[1] 朱燕[1] 章宗穰[1] 朱建中[2] 张国雄[2] 

机构地区：[1]上海师范大学化学系,上海200234 [2]中国科学院上海冶金研究所,上海200050

出　　处：《分析化学》2000年第7期911-914,共4页Chinese Journal of Analytical Chemistry

基　　金：上海市教委发展基金

摘　　要：采用微机械加工技术制作了具有三维结构的微腔型传感器芯片（腔深３００μｍ，铂工 作电极１．２×１．２ｍｍ２）。作者对微腔型电极进行了电化学特性的表征。并通过明胶包埋法将 半乳糖氧化酶固定在电极中，构成微腔型半乳糖传感器。该酶电极对半乳糖测定的上限为 ４．５ｍｍｏｌ／Ｌ，相关系数为０．９９８０。较适宜的ｐＨ范围为７．０～７，５，响应时间＜５０ ｓ。该酶电极 在１４天内连续使用，响应电流无明显变化。放置一个月后测试仍保持较好的电流响应。A microchip with three dimensional silicon chamber (l. 2 x 1 .2 mm2 and 300 μm thick) was developed for the construction of micro-size biosensor. The electrochemical characterization of its Pt working electrode was reported. A galactose biosensor was constructed with the substrate working electrode of chamber-type microchip. The upper detection limit of the microsensor is 4. 5 mmol/L and the response time was less than 50 s. No significant variation was observed in detection within two weeks.

关 键 词：半乳糖传感器 微控型电极 半乳糖测定 

分 类 号：TP212[自动化与计算机技术—检测技术与自动化装置]