DNA修饰碱基5-甲基胞嘧啶和8-羟基鸟嘌呤的毛细管气相色谱分析及质谱鉴定  

Capillary Gas Chromatographic Analysis and Mass Spectrometric Identification of Modified DNA Bases 5-Methylcytosine and 8-Hydroxyguanine

作　　者：宋元宗[1] 祝其锋[1] 庄海旗[2] 莫丽儿[2] 

机构地区：[1]广东医学院医用生化研究所,广东湛江524023 [2]广东医学院化学教研室,广东湛江524023

出　　处：《色谱》2000年第4期295-299,共5页Chinese Journal of Chromatography

基　　金：广东省重点学科资助!课题;批准号 :粤高教科 [1 997]1 4号

摘　　要：探索了GC/FID定量分析DNA修饰碱基 5 甲基胞嘧啶和 8 羟基鸟嘌呤的实验条件 ,用GC/MS鉴定各有关成分。结果表明 ,DNA水解物中不同成分可被成功地衍生和分离 ;5 甲基胞嘧啶和 8 羟基鸟嘌呤的相对摩尔反应因子分别为 3 0和 1 3;灵敏度分别为 5 50× 1 0 9mV·s/ g和 7 59× 1 0 1 0 mV·s/ g ;检测限可分别达 36 4pg/s和 1 5 8pg/s ;整个分析流程的相对标准偏差小于 2 0 %。The purpose of this paper was to establish a method for the determination of modified bases, 5 methylcytosine and 8 hydroxyguanine,in DNA by GC/FID The experimental conditions were explored systematically for the quantitative analysis of these two modified bases, and the components were identified by GC/MS The results showed that the variant components in DNA treated with Fenton’s reaction can be derivatized and separated successfully. The relative molar reactive factors of 5 methylcytosine and 8 hydroxyguanine were 3 0 and 1 3, respectively. The sensitivity for them were 5 50×10\+9?mV·s/g and 7 59×10\+\{10\}?mV·s/g, respectively,while their detectable limits were 36 4?pg/s and 15 8?pg/s,respectively. The coefficients of variation for gas chromatograph were less than 5%, for derivatization, less than 6%,and for the whole analysis process ,less than 20%

关 键 词：5-甲基胞嘧啶 8-羟基鸟嘌呤 GC/FID 分析鉴定 

分 类 号：Q526.03[生物学—生物化学] O657.71[理学—分析化学]