高效液相色谱和质谱法对产酸克雷伯氏菌SG-11生物合成吲哚-3-乙酸代谢途径的研究  

Research of Indole-3-Acetic Acid Biosynthetic Pathway of Klebsiella oxytoca SG-11 by HPLC and GC-MS

作　　者：吕泽勋[1] 宋未[1] 

机构地区：[1]中国农业科学院原子能利用研究所,北京100094

出　　处：《色谱》2000年第4期328-331,共4页Chinese Journal of Chromatography

基　　金：国家自然科学基金!项目 ( 39570 0 2 5);农业部"九五"基础研究项目! ( 95农 1 6 0 3 0 2 )

摘　　要：产酸克雷伯氏菌 (Klebsiellaoxytoca)SG 1 1是从水稻根面分离的植物根际促生细菌 ,具有生物固氮能力。利用HPLC和GC MS对该菌的代谢产物进行了定性定量分析 ,结果表明该菌能产生较高浓度的吲哚 3 乙酸(IAA) ;对其代谢途径的研究结果证明 ,该菌以吲哚 3 丙酮酸代谢途径合成IAA。The plant growth promoting bacteria are closely associated to plant The bacteria are used to adhering to plant rhizoplane, promoting plant growth by fixing nitrogen from atmosphere, secreting stimulating substances or producing antagonistic to plant pathogens It was indicated that the biological nitrogen fixation played an important role in plant growth promoting function In fact, it was verified recently by overall research that IAA does it.Therefore research of IAA production and biosynthetic pathway of plant growth promoting bacteria is much more important The various ways of IAA production indicated the strong or weak promoting function of bacterium to plants in general The purpose of this paper is to determine whether IAA exists in cultured medium of Klebsiella oxytoca SG 11 and biosynthetic pathway of IAA, in order to opimize cultural conditions for IAA production \;Klebsiella oxytoca SG 11 is a plant growth promoting bacterium, isolated from rice rhizoplane, which can fix nitrogen The supernatant of SG 11 cultured medium determined by HPLC showed that 47 4 mg/L of IAA existed in LB medium and 1 2 mg/L of IAA, in basal medium IAA in metabolite was identified by GC/MS as well The intermediate determination of tryptamine, indole 3 acetamide, tryptophol and indole 3 acetonitrile indirectly indicated that IAA was biosynthesized in a pathway of indole 3 pyruvic acid Meanwhile, tryptophol in metabolite of SG 11 was verified by GC/MS The direct intermediates of indole 3 pyruvic acid and indole 3 acetaldehyde in the pathway can not be determined, because both are unstable under normal condition As reversible conversion existed between indole 3 pyruvic aldehyde and tryptophol, the presence of tryptophol also proved the pathway of indole 3 pyruvic acid in the synthesis of IAA by Klebsiella oxytoca SG 11. The results laid basis for further research of plant growth promoting function of the bacterium.

关 键 词：产酸克雷伯氏菌SG-11 吲哚-3-乙酸 代谢途径 

分 类 号：Q946.885.1[生物学—植物学] Q945.13