杂种鹅掌楸体胚苗根尖染色体核型分析  

Karyotype analysis of the Liriodendron chinense×L.tulipifera root tip chromosome

作　　者：刘光欣[1] 陈金慧[1] 孙杰[1] 愈天驰[1] 施季森[1] 

机构地区：[1]南京林业大学,林木遗传与生物技术省部共建教育部重点实验室,江苏南京210037

出　　处：《南京林业大学学报（自然科学版）》2012年第6期13-16,共4页Journal of Nanjing Forestry University：Natural Sciences Edition

基　　金：国家林业局“948”引进项目(2009-4-24);江苏省自然科学基金项目攀登计划(BK2008051);国家自然科学基金项目(30901156);江苏省高校自然科学基金项目(09KJA220001)

摘　　要：分别采用冰水混合物、0.7 mmol/L环己酰胺和0.2%秋水仙素3种试剂,在多个时间梯度对生长旺盛的杂种鹅掌楸体胚苗根尖进行预处理,并采用压片法进行制片观察。结果表明:采用冰水混合物处理6 h,0.7 mmol/L环己酰胺处理2 h,0.2%秋水仙素处理4 h,均可获得较多的根尖细胞中期分裂相。相比较而言,采用环己酰胺和秋水仙素作为预处理试剂获得的染色体形态较好。利用0.7 mmol/L环己酰胺对鹅掌楸根尖染色体进行2 h的预处理后,采用卡宝品红压片法制片进行染色体核型分析的结果显示,杂种鹅掌楸染色体为38条,核型公式为2n=38=26m+8sm+2st+2t,核型属于2B型。Ice-water mixture,0.7 mmol/L cycloheximide and 0.2 % colchicine were used as pretreated reagent for Liriodendron hybrids root tips of test-tube plantlet by different time grads.Then the root tips were squashed with Carbol fuchsin and observed with microscope.It had been proved that all these three pretreatments of ice-water mixture for 6 h,0.7 mmol/L cycloheximide for 2 h,and 0.2 % colchicine for 4 h could obtain metaphase chromosome specimen,but by comparison,root tips pretreated with cycloheximide and colchicine could give much better chromosome morphology.Then Karyotype of the Liriodendron chinense×L.tulipifera was studied with chromosome samples prepared from root tip by 0.7 mmol/L cycloheximide for 2 h.The result showed that there were 38 chromosome in Liriodendron chinense×L.tulipifera and its karyotype formula was 2n=38=26m＋8sm＋2st＋2t and the karyotypes of Liriodendron chinense×L.tulipifera belong to category 2B of Stebbins.

关 键 词：杂种鹅掌楸 染色体 核型分析 

分 类 号：Q943[生物学—植物学]