盐胁迫下水杨酸对南林895杨组培苗抗氧化系统的影响  

Effects of salicylic acid on oxidation resistance of 'Nanlin 895' poplar plantlets in vitro under salt stress

作　　者：陈颖[1] 徐彩平[1] 汪南阳[1] 胡菲[1] 王光萍[1] 黄敏仁[1] 

机构地区：[1]南京林业大学森林资源与环境学院,江苏省杨树种质创新与品种改良重点实验室,江苏南京210037

出　　处：《南京林业大学学报（自然科学版）》2012年第6期17-22,共6页Journal of Nanjing Forestry University：Natural Sciences Edition

基　　金：江苏省自然科学基金项目(BK2012818);江苏省杨树种质创新与品种改良重点实验室开放课题(2010);江苏高校优势学科建设工程资助项目(PAPD)

摘　　要：以南林895杨组培苗为材料,研究了盐胁迫下水杨酸(SA)对其耐盐及抗氧化能力的影响。结果表明:与单独盐处理(100 mmol/L NaCl)相比,盐胁迫下添加SA(0.01、0.05、0.1 mmol/L)能显著地提高南林895杨苗的含水量和总生物量;显著提高根和叶中超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、过氧化物酶(POD,叶中除外)、抗坏血酸过氧化物酶(APX)、谷胱甘肽还原酶(GR)等5种抗氧化酶活性和抗坏血酸(AsA)、还原型谷胱苷肽(GSH)两种抗氧化剂的含量,其中0.01和0.05 mmol/L SA处理对保护酶提高效果显著;而0.05和0.1 mmol/L SA处理显著地降低了根和叶中O.2-和H2O2的含量,且在根中降低的程度远大于叶片中,另外SA处理也显著降低了叶片中MDA的含量。总之,水杨酸主要通过提高南林895杨根和叶中保护酶活性、增加抗氧化剂含量来清除活性氧,根和叶中对活性氧的清除存在不同的机制,抗坏血酸-谷胱甘肽循环参与了叶片中H2O2的清除,而根中H2O2的清除主要由过氧化物酶和抗坏血酸过氧化物酶来完成。0.01～0.05 mmol/L的水杨酸可以有效缓解盐胁迫对南林895杨组培苗的伤害。The effect of salicylic acid（SA） supply（0.01,0.05,0.1 mmol/L） on the antioxidant resistance and changes of reactive oxygen species（ROS） in ＇Nanlin 895＇ poplar plantlets（Populus delotides×P.euramericana cv.＇Nanlin 895＇） in vitro was investigated under treatment 100 mmol/L NaCl.The results showed that exogenous SA significantly enhanced five antioxidant enzymes activities including superoxide dismutase（SOD）,catalase（CAT）,peroxidase（POD） except in leaves,ascorbate peroxidase（APX） and glutathione reductase（GR）,and also increased the content of ascorbic acid（AsA） and reduced glutathione（GSH）.However SA significantly alleviated active oxygen damage（e.g.O-· 2,H2O2） in leaves and roots,reduced the generation of MDA in leaves.These results suggest that exogenous SA could improve activity of protective enzymes,and ameliorate the negative effect of salinity on poplar growth.Poplar roots and leaves showed different mechanisms of scavenging ROS during the application of SA under salt stress.The ascorbate-glutathione pathway is related to scavenging H2O2 in polar leaves while peroxidase are responsible for scavenging H2O2 in poplar roots.0.01-0.05 mmol/L SA treatments could obviously alleviate salinity injury of ＇Nanlin 895＇ poplar plantlets.

关 键 词：水杨酸 盐胁迫 南林895杨组培苗 抗氧化系统 

分 类 号：S718[农业科学—林学]