杏仁核点燃癫痫大鼠模型海马组织突触囊泡蛋白2A的表达变化  

Expression changes and significance of SV2A in the hippocampus of rats with amygdala-kindling epilepsy models

作　　者：毛诗贤[1] 冯亚梅[1] 楚兰[1] 郭文洁[1] 李洁[1] 

机构地区：[1]贵阳医学院附属医院神经内科,贵州贵阳550004

出　　处：《中风与神经疾病杂志》2012年第11期1008-1011,共4页Journal of Apoplexy and Nervous Diseases

摘　　要：目的探讨杏仁核点燃癫痫发生过程中突触囊泡蛋白2A(synaptic vesicle protein 2A,SV2A)在海马组织中表达的动态变化。方法建立杏仁核点燃癫痫大鼠模型,治疗组予左乙拉西坦(levetiracetam,LEV)灌胃,用荧光定量PCR(RealTime-PCR)测定不同时期SV2A mRNA的表达,免疫组织化学方法检测SV2A蛋白的表达。结果 SV2A在1w时表达降低,之后逐渐升高,到8w时达高峰,与对照组比较差异有统计学意义(P<0.05);用药前后相比,除1w组外,2w组、4w组、8w组用相比差异有统计学意义(P<0.05)。结论 SV2A的表达与癫痫的发作有关。Objective To establish the amygdala-kindling epilepsy rats models,and measure the expression of SV2A to explore the pathogenesis of epilepsy.Methods To establish model of chronic epilepsy by stimulating the amygdala as the epilepsy group,and to perfuse antiepileptic drug of levetiracetam into stomach as the treatment group.The dynamic changes of SV2A were examined by Real-time PCR and immunohistochemistry in the hippocamp tissue.Results Compared with the sham control group,epilepsy group showed decreased the expression after one week,and SV2A in epilepsy eight weeks was higher than control group（P0.05）.However,there was significant difference between group after two weeks,four weeks and eight weeks before and after treatment（P0.05）.Conclusion The expression of SV2A was closely related to epileptic seizures.

关 键 词：突触囊泡蛋白2A 海马 慢性癫痫 左乙拉西坦 

分 类 号：R742.1[医药卫生—神经病学与精神病学]