消痞颗粒对慢性萎缩性胃炎伴不典型增生大鼠胃黏膜增殖和凋亡的影响  

Effect of Xiaopi Granules on Proliferation and Apoptosis in the Gastric Mucosa of Chronic Atrophic Gastritis Rats with Atypical Hyperplasia

作　　者：赵晶[1] 孟捷[2] 杨晋翔[3] 彭继升[3] 

机构地区：[1]浙江大学生命科学学院 [2]北京中医药大学东方医院 [3]北京中医药大学第三附属医院

出　　处：《中医杂志》2012年第24期2118-2121,共4页Journal of Traditional Chinese Medicine

基　　金：教育部2009年度"高等学校博士学科点专项科研基金"资助项目(20090013110002)

摘　　要：目的探讨慢性萎缩性胃炎伴不典型增生的发病机制及消痞颗粒对其作用的机制。方法将60只Wistar大鼠随机分为正常组、模型组、自然恢复组、消痞颗粒组、维酶素组,每组12只。采用幽门弹簧插入术加灌胃高盐热糊法复制大鼠模型,正常组、模型组于第16周造模成功后处死;消痞颗粒组大鼠于手术1周后,给予浓度为0.6g/ml的消痞颗粒溶液灌胃0.5ml/(100g.d);自然恢复组大鼠于造模16周后,蒸馏水灌胃0.5ml/(100g.d);维酶素组大鼠手术1周后,以浓度为0.4g/ml维霉素混悬液0.5ml/(100g.d)灌胃,各组均每周灌胃6天,12周后采用免疫组化法检测胃黏膜增殖细胞核抗原(PCNA)、B细胞淋巴瘤/白血病-2基因(Bcl-2)和Bcl-2相关X蛋白(Bax)表达。结果模型组与自然恢复组PCNA阳性细胞面密度、Bcl-2均明显高于正常组(P<0.05或P<0.01);Bax低于正常组(P<0.05或P<0.01);与模型组比较,其余各组PCNA阳性细胞面密度差异均有统计学意义(P<0.05或P<0.01)。消痞颗粒组PCNA面密度明显低于自然恢复组(P<0.05),消痞颗粒组与维酶素组比较差异无统计学意义(P>0.05)。消痞颗粒组Bcl-2较模型组明显减弱(P<0.05),Bax明显升高(P<0.01);消痞颗粒组与维酶素组比较差异均无统计学意义(P>0.05)。结论降低胃癌癌前病变大鼠胃黏膜中的PCNA、Bcl-2表达、升高Bax表达可能是消痞颗粒改善慢性萎缩性胃炎伴不典型增生有效机制之一。Objective To observe the therapeutic effect and mechanism of Xiaopi Granules （granules to disperse epigastric fullness syndrome） on chronic atrophic gastritis （CAG） with atypical hyperplasia （ATP）. Methods Sixty male wistar rats were randomized into normal group, model group, natural recovery group, Xiaopi Granules group and vitacoenzyme group, with 12 in each. The rat models were replicated by methods of pylorus spring insertion and high-salt hot paste for 16 weeks. The rats in the normal group and the model group were executed after 16 weeks of modeling. The rats in the Xiaopi Granules group were intragastrically administrated with 0.5ml/（100g ~ d） of Xiaopi Granules one week after surgery. The rats in the natural recovery group were intragastrically administrated with 0.5ml/（100g ~ d） of distilled water after 16 weeks of modeling. The rats in the vitacoenzyme group were intragastrically administrated with 0.5ml/（100g ~ d） （0.04g/ml） of vitacoenzyme sus- pension one week after surgery. The intragastric administration was given 6 times weekly for 12 weeks in all groups. The ex- pressions of proliferation cell nuclear antigen （PCNA）, Bcl-2 and Bax in the gastric mucosa were detected with immunohisto- chemical method. Results The positive expressions of PCNA and Bel-2 were significantly increased and the positive expression of Bax was significantly decreased in the model group and the natural recovery group compared with the normal group （P~ 0.05 or P^0.01）. Compared with the model group, the positive expression density of PCNA was significantly different in all other groups （P^0.05 or P^0.01）. The positive expression density of PCNA in the Xiaopi Granules group was significantly lower than that in the natural recovery group （P^0.05） and has no significant difference with the vitacoenzyme group （P~ 0.05）. The expression of Bcl-2 was reduced and the expression of Bax was increased significantly （P^0.01） in the Xiaopi Granules group as compared with those in the mo

关 键 词：消痞颗粒 慢性萎缩性胃炎 不典型增生 增殖细胞核抗原 Bcl-2 BAX 凋亡 增殖 

分 类 号：R259[医药卫生—中西医结合]