900 MHz微波辐射对雄性大鼠血浆激素水平昼夜节律的影响  

Effects of 900 MHz Microwave Radiation on Circadian Rhythm of Plasma Hormone Level in Male Rats

作　　者：赵华[1] 宗春燕[1] 周振[1] 姜秉成[1] 胡文珠[1] 童建[1] 曹毅[1] 

机构地区：[1]苏州大学公共卫生学院,江苏苏州215123

出　　处：《环境与职业医学》2012年第12期735-738,共4页Journal of Environmental and Occupational Medicine

基　　金：973项目资助课题(编号:2011CB503700)

摘　　要：[目的]观察900MHz微波辐射对雄性大鼠血浆褪黑素、睾酮和雌二醇水平昼夜节律的影响。[方法]健康雄性SD大鼠30只,适应性喂养30d后采集大鼠24h自发活动数据,利用生物节律单余弦分析软件筛选具有昼夜节律的大鼠17只,再对该17只大鼠24h采血6次测血浆褪黑素水平,然后选取褪黑素水平昼夜节律差异无统计学意义的大鼠10只,随机分为对照组和照射组,每组5只。照射组在昼夜时点(ZT)1点给予2000μW/cm2900MHz的微波辐射,1h/d,持续30d;对照组除不给予微波辐射外,其他条件同照射组(即假照射)。大鼠在辐射结束后第0、4、8、12、16、20h各采血一次,测定大鼠血浆的褪黑素、睾酮和雌二醇水平,比较照射组与对照组血浆褪黑素、睾酮和雌二醇水平昼夜节律的不同。[结果]对照组在假照射后血浆褪黑素水平、睾酮水平存在明显昼夜节律,照射组在微波辐射后血浆褪黑素水平和睾酮水平也存在明显昼夜节律,但微波辐射后相位响应曲线的中值、振幅、峰值时点与对照组假照射后相比发生了明显变化(P<0.05)。对照组假照射后血浆雌二醇水平有明显昼夜节律(P<0.05),照射组微波辐射后血浆雌二醇水平不存在昼夜节律(P>0.05)。[结论]2000μW/cm2900MHz微波辐射可影响雄性大鼠血浆褪黑素、睾酮和雌二醇水平的昼夜节律。[ Objective ] To investigate the effects of 900 MHz microwave radiation on circadian rhythm of melatonin, testosterone, and estradiol leveis in plasma of male rats. [ Methods ] The locomotor activity of 30 adult male SD rats were measured after 30d of adaption and fitted to a single cosine curve with a 24h cycle for circadian rhythm screening. After that 10 out of 17 rats with similar circadian rhythm of melatonin levels in plasma were randomly divided into a control group and a radiation group （5 rats for each group）. The latter was exposed to microwave radiation （2 000 μW/cm^2, 900 MHz, 1 h/d, 30 d） at zeitgeber time （ZT） 1. The control group was treated in the same condition of the radiation group except radiation. Blood samples were collected from caudal vein every 4h after the last radiation exposure. Testosterone, estradiol, and melatonin levels in plasma of blood samples were determined and fitted to phase response curves （PRCs） using a cosine model.[ Results ] The mesor, amplitude, and acrophase of PRCs for plasma melatonin level and testosterone level showed significant differences between the control group and the radiation group （P〈0.05）. The plasma estradiol levels presented significant circadian rhythm in the control group, but not in the radiation group. [ Conclusion ] The findings indicate that 2000μW/cm^2, 900MHz microwave radiation can affect the circadian rhythm of plasma testosterone, estradiol, and melatonin levels in male rats.

关 键 词：微波辐射 昼夜节律 褪黑素 睾酮 雌二醇 

分 类 号：R114[医药卫生—卫生毒理学]