电子线路板粉末对雄性小鼠的毒性作用  

Oral Toxicity of Circuit Board Powder to Male Mice

作　　者：高明[1] 吴南翔[1] 宋鹏[1] 楼建林[1] 宋杨[1] 

机构地区：[1]浙江省医学科学院卫生学研究所,浙江杭州310013

出　　处：《环境与职业医学》2012年第12期767-770,共4页Journal of Environmental and Occupational Medicine

基　　金：浙江省科技厅专项(编号:2008C03001-1;2007C30015)

摘　　要：[目的]研究电子线路板粉末喂饲雄性小鼠,对其脏器尤其是生殖系统的毒性作用。[方法]雄性小鼠分别喂饲常规饲料和含电子线路板粉末的混合饲料,定期记录体质量和摄食量,90d后处死,取脑、心、肝、脾、肺、肾和睾丸称重并进行病理切片观察,计算脏器系数,测定肝、脑中多溴联苯醚(PBDEs)的含量,测定精子活力及相关动力学参数,免疫荧光法观察睾丸组织Connexin43蛋白表达。[结果]电子线路板粉末24h浸出液致小鼠急性半数致死量(LD50)大于10000mg/kg。经过90d亚慢性实验,实验组小鼠主要脏器系数[肝(4.63±0.39)、肾(1.72±0.29)、脑(1.02±0.13)、肺(0.51±0.04)]比对照组相应脏器系数[肝(3.99±0.42)、肾(1.38±0.16)、脑(0.85±0.15)、肺(0.46±0.06)]明显升高,并且肝、肾出现病理损伤。实验组肝、脑中PBDEs含量分别为175.54ng/g湿重、29.60ng/g湿重,较对照组肝(2.16ng/g湿重)、脑(0.12ng/g湿重)大幅上升。实验组和对照组相比,睾丸的脏器系数、病理切片和Connexin43蛋白表达均未出现明显改变,精子活力及相关动力学参数差异也未见统计学意义。[结论]成年雄性小鼠喂饲电子线路板粉末90d后,肝脏和肾脏出现明显病理学改变,生殖系统未观察到毒性效应。[ Objective ] To study the toxicity of circuit board powder on main organs, especially on reproductive system of male mice by oral feeding. [ Methods ] Male ICR mice were fed with either normal diet or the mixed diet containing circuit board powder. Body weight and food intake were recorded regularly. After 90 days of dietary treatment, the mice were sacrificed, and the organs （brain, heart, liver, spleen, lung, kidney, and testis） were weighed and histopathologically examined. Organ coefficients were calculated. Levels of polybrominated diphenyl ethers （PBDEs） in liver and brain were determined. Motility and kinetic parameters of sperm were measured. Expression of Connexin43 protein in testis was observed by immunofluorescence. [ Results ] The acute oral lethal dose （LD50） of male mice after treatment of 24h lixivium of circuit board powder was greater than 10 000 mg/kg. After a 90d subchronic experiment, the organ coefficients of liver （4.63 ± 0.39）, kidney （1.72 ± 0.29）, brain （1.02 ± 0.13）, and lung （0.51 ± 0.04） in the test group were significantly increased when compared with those in the control group （3.99 ± 0.42, 1.38 ± 0.16, 0.85 ± 0.15, 0146 ± 0.06, respectively）. Pathological damage was found in the liver and kidney of the tested mice. The PBDEs levels in liver （175.54ng/g wet weight） and brain （29.60ng/g wet weight） of the test group were significantly increased when compared with those （liver, 2.16 ng/g wet weight; brain, 0.12 ng/g wet weight） of the control group, respectively. No obvious changes in the organ coefficient, pathological section, and expression of Connexin43 protein were observed in the testis of both the test and control groups. There were no significant differences in the motility and kinetic parameters of sperm between the test and control groups. [ Conclusion ] Pathological changes are found in the liver and kidney of adult male mice after 90-day diet containing circuit board powder, while no reproductive toxic effect is observed

关 键 词：电子线路板粉末 脏器系数 生殖系统 精子活力 毒性 小鼠 

分 类 号：R114[医药卫生—卫生毒理学]