活性氧在芬太尼后处理和肢体远隔缺血后处理减轻大鼠心肌缺血再灌注损伤中的作用  

Roles of reactive oxygen species in the reduction of myocardial ischemia-reperfusion injury by fentanyl postconditioning and remote limb ischemic postconditioning in rats

作　　者：许亚超[1] 薛富善[2] 袁玉静[2] 王强[2] 廖旭[2] 程怡[2] 李瑞萍[2] 刘建华[2] 王天龙[1] 

机构地区：[1]首都医科大学宣武医院麻醉科,北京市100053 [2]中国医学科学院北京协和医学院整形外科医院麻醉科

出　　处：《中华麻醉学杂志》2012年第10期1257-1261,共5页Chinese Journal of Anesthesiology

基　　金：国家自然科学基金（30972836）

摘　　要：目的探讨活性氧在芬太尼后处理和肢体远隔缺血后处理减轻大鼠心肌缺血再灌注损伤中的作用。方法成年雄性sD大鼠63只，8周龄，体重250～350g，采用随机数字表法，将其随机分为7组（n=9）：假手术组（s组）、心肌缺血再灌注组（I／R组）、芬太尼后处理组（F组）、肢体远隔缺血后处理组（R组）、活性氧清除剂N．（2．巯基丙酰基）一甘氨酸（MPG）组（M组）、MPG＋芬太尼后处理组（MF组）和MPG＋肢体远隔缺血后处理组（MR组）。除s组外，其余6组采用结扎左冠状动脉前降支30min再灌注180min的方法制备心肌缺血再灌注损伤模型。在缺血前5min至再灌注15minM组、MF组和MR组静脉输注MPG5mg／kg，其他4组给予等容量生理盐水，心肌缺血15min时，F组和MF组静脉注射芬太尼30μg／kg；R组和MR组结扎大鼠双后肢造成缺血10min。心肌再灌注180min时，采集动脉血样，测定血清心肌肌钙蛋白I（cTnI）浓度，然后处死大鼠，取心肌组织，采用TTC染色法测定心肌梗死体积。结果与s组比较，其余6组血清cTnI浓度和心肌梗死体积均升高（P〈0．05）；与I／R组比较，M组血清crrnI浓度和心肌梗死体积差异无统计学意义（P〉0．05），F组和R组血清cTnI浓度和心肌梗死体积降低（P〈0．05）；MF组和F组血清cTnI浓度和心肌梗死体积差异无统计学意义（P〉0．05）；MR组血清cTnI浓度和心肌梗死体积高于R组（P〉0．05）。结论活性氧参与了肢体远隔缺血后处理减轻大鼠心肌缺血再灌注损伤的效应，而未参与芬太尼后处理的心肌保护作用。Objective To investigate the role of reactive oxygen species （ROS） in the reduction of myocardial ischemia-reperfusion （I/R） injury by fentanyl postconditioning and remote limb ischemic postconditioning in rats. Methods Sixty-three male Sprague-Dawley rats, aged 8 weeks, weighing 250-350 g, were equally and ran- domly allocated into 7 groups： sham operation group （group S）, group I/R, fentanyl postconditioning group （group F）, remote limb ischemic postconditioning group （group R）, ROS scavenger N-（2-Mercaptopropionyl） glycine （MPG） group （group M）, MPG ＋ fentanyl postconditioning group （group MF）, and MPG ＋ remote limb isch- emic postconditioning group （group MR）. Myocardial I/R was induced by occlusion of anterior descending branch of left coronary artery for 30 rain followed by 180 rain of reperfusion. In group S the anterior descending branch was only exposed but not ligated. MPG 5 mg/kg was infused intravenously from 5 min before ischemia to 15 min of reperfusion in groups M, MF and MR, while the equal volume of normal saline was given in the other four groups.In groups F and MF, fentanyl 30μg/kg was injected intravenously at 15 min of myocardial ischemia. In groups R and MR, the animals underwent＂ 10 min ischemia of bilateral hind limbs starting from 15 min of myocardial ischemia. Arterial blood samples were taken at 180 min of reperfusion to determine the serum cardiac troponin I （cTnI） concentration. The rats were then sacrificed. The infarct size was measured by TTC. Results Compared with group S, the serum cTnI concentration and infarct size were significantly increased in the other six groups （ P 〈 0.05 ）. Compared with group I/R, no significant change was found in the serum cTnI concentration and infarct size in M group, and the serum cTnI concentration and infarct size were significantly decreased in Fand R groups （P 〈 0.05）. There was no significant difference in the serum cTnI concentration and infarct size between MF group and F group

关 键 词：活性氧 芬太尼 下肢 心肌再灌注损伤 缺血后处理 

分 类 号：R614[医药卫生—麻醉学]