Co(phen)_2TATP^(3+)与DNA在旋转金盘金环电极上的相互作用研究  

Electrochemical Studies on the Interactions of the Complex of Co(phen)_2TATP^(3+) with DNA at Rotating Au Au Electrode

作　　者：李红[1] 蒋雄[2] 王雷[3] 叶保辉[1] 计亮年[1] 

机构地区：[1]中山大学化学与化工学院,广州510275 [2]华南师范大学化学系,广州510631 [3]广州师范学院化学系,广州510405

出　　处：《高等学校化学学报》2000年第7期995-998,共4页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金! (批准号 :2 98710 36);广东省自然科学基金!(批准号 :970 2 11)资助

摘　　要：在pH =7.2的Tris缓冲溶液中 ,利用旋转环盘电极法研究了金电极上Co(phen) 2 TATP3 + 与DNA的相互作用 ,并根据扩散控制和电化学控制下得到的各种参数 ,对它们作用的模式进行了讨论 .发现当一定量的DNA存在时 ,Co(phen) 2 TATP3 + 的扩散系数、还原反应的传递系数和半波电位下的速率常数、还原产物Co(phen) 2 TATP2 + 的收集系数和脱出率等都发生了较大幅度的减小 .利用Co(bpy) 3 + 3 进行比较研究表明 ,Co(phen) 2 TATP3 + 与Co(bpy) 3 + 3 在加入DNA后在收集系数和传递系数变化上存在较大的差异 .The interaction between complex Co(phen) 2TATP 3+ (phen=1,10 phenanthroline, TATP= 1,4,8,9 tetranitrogen trisphene) and DNA has been investigated by using rotating ring disk electrode technique, and its models are discussed from the parameters obtained under diffusion and electrochemical control, respectively. It has been found that the values of the following electrochemical parameters: the apparent diffusion coefficient, transfer coefficient, rate constant at half wave potential, collection coefficient, desorption efficiency etc., for the complex of Co(phen) 2TATP 3+ decrease significantly in the presence of DNA compared with that in the absence of DNA. It is shown from the comparative studies of Co(bpy) 3+ 3 that, in the presence of DNA, significant differences exist in the change of transfer coefficient and collection coefficient between Co(bpy) 3+ 3 and Co(phen) 2TATP 3+ .

关 键 词：多吡啶钴(Ⅲ)配合物 DNA 金电极 相互作用 

分 类 号：Q523[生物学—生物化学] O614.812[理学—无机化学]