部队医疗机构室内空气中细菌抗消毒剂基因qacEΔ1检测  

Detection of disinfectant-resistant gene of qacEΔ1 in bacteria of indoor air of some military medical institutions

作　　者：杨银书[1] 李强[1] 第五进学[1] 赵霞[1] 赵瑞阳[1] 崔明[1] 石映祥[1] 

机构地区：[1]兰州军区疾病预防控制中心消杀灭科,甘肃兰州730020

出　　处：《中华医院感染学杂志》2012年第24期5458-5459,共2页Chinese Journal of Nosocomiology

摘　　要：目的研究西北地区东片部队医疗机构室空气细菌抗消毒剂基因qacEΔ1的阳性率,为部队医疗机构空气消毒及医院感染预防治疗提供指导依据。方法用PCR方法检测空气中分离的细菌的qacEΔ1基因。结果 98株细菌中有23株携带抗消毒剂基因qacEΔ1,阳性率为23.5%;大肠埃希菌、铜绿假单胞菌、金黄色葡萄球菌、肺炎克雷伯菌、表皮葡萄球菌qacEΔ1的检出率分别为36.7%、36.4%、27.8%、20.0%、10.0%;白色假丝酵母菌、蜡样芽胞杆菌、枯草芽胞杆菌黑色变种芽胞、流感嗜血菌未检出qacEΔ1基因。结论西北地区东片部队医疗机构空气中的细菌抗消毒剂基因qacEΔ1阳性率不高,但应注意消毒剂的交替使用。OBJECTIVE To study the positive rate of disinfectant-resistant gene of qacEΔ1 in bactria of indoor air of military medical institutions in east part of Northwest China so as to provide basis for the disinfection of air of military medical institutions and preuention of nosocorni as infections.METHODS Gene of qacEΔ1 in the bacteria isolated from the indoor air was detected by PCR.RESULTS Gene of qacEΔ1 was found in 23 strains of bacteria among 98 strains of bacteria,the total positive rate was 23.5%.The positive rates of gene of qacEΔ1 in Escherichia coli,Pseudomonas aeruginosa,Staphylococcus aureus,Klebsiella pneumoniae,S.epidermidis were respectively 36.7%,36.4%,27.8%,20.0%,and 10.0%;and the gene of qacEΔ1 was not found in Candida albicans,Bacillus cereus,black variant spores of Bacillus subtilis,and Haemophilus influenzae.CONCLUSION Though the positive rate of gene of qacEΔ1 in bacteria of indoor air in military medical institutions in east part of Northwest China is not high,yet we should focus on the alternate use of disintectant.

关 键 词：医疗机构 抗消毒剂基因qacEΔ1 检测 

分 类 号：R378[医药卫生—病原生物学]