厌氧血培养对疑似血流感染患者临床诊断治疗的影响  

Effect of anaerobic blood culture on diagnosis and treatment of suspected bloodstream infections

作　　者：余世全[1] 周惠琴[2] 刘励军[1] 

机构地区：[1]苏州大学附属第二医院ICU2,江苏苏州215004 [2]苏州大学附属第二医院医院细菌室,江苏苏州215004

出　　处：《中华医院感染学杂志》2012年第24期5670-5672,共3页Chinese Journal of Nosocomiology

摘　　要：目的探讨厌氧血培养对疑似血流感染患者血培养阳性率的影响,为临床及时正确的诊断及治疗提供依据。方法血液标本采用双套需氧瓶加厌氧瓶血培养,用BACTEC-9120全自动血培养仪进行检测后用VITEK-32仪器及鉴定卡分析鉴定。结果血培养送检标本1744份,阳性215份,阳性率为12.33%,分离出菌株80株;需氧血培养、厌氧血培养均阳性为84份,占39.07%,需氧血培养阳性、厌氧血培养阴性为111份,占51.63%,需氧血培养阴性、厌氧血培养阳性为20份,占9.30%,需氧血培养及厌氧血培养结果比较,差异有统计学意义(χ2=23.536,P<0.05);需氧血培养及厌氧血培养均阳性的标本中,5株病原菌为厌氧血培养提前2d出现阳性,2株为提前1d出现阳性。结论厌氧血培养可提高疑似血流感染患者血培养的阳性率;双套需氧血培养加厌氧血培养模式可提高血培养阳性率,更早期得出培养结果,避免不必要的漏检,可对疾病进行及时正确的诊断及治疗,对提高患者的治愈率有重要的作用。OBJECTIVE To study the effect of anaerobic blood culture on the positive rate of blood culture of the patients with suspected bloodstream infections so as to provide basis for the timely diagnosis and treatment.METHODS The blood sample were cultured by adopting two sets of aerobic and anaerobic blood culture bottles,the culture result was identified and analyzed by using VITEK-32 instrument and identification card after the detection with BACTEC-9120 full-automatic blood culture system.RESULTS Of 1744 blood culture samples submitted,there were 215 positive with the positive rate of 12.33%,totally 80 strains of bacteria were isolated;there were 84 both aerobic and anaerobic culture positive,accounting for 39.07%,and there were 111 aerobic culture positive with anaerobic culture negative,accounting for 51.63%,and there were 20 aerobic culture negative with anaerobic culture positive,accounting for 9.30%,as compared with the result of the aerobic and anaerobic blood cultures,the differences were statistically significant（χ2=23.536,P0.05）;of the both aerobic and anaerobic blood culture positive samples,there were 5 strains of pathogens appeared as positive 2 days before the anaerobic blood culture,2 strains as positive 1 day before the anaerobic blood culture.CONCLUSION The anaerobic blood culture can increase the positive rate of the blood culture of the patients with suspected bloodstream infections;two sets of aerobic and anaerobic blood culture mode can improve the positive rate of blood culture,ensure the acquirement of the culture result in the early stage,avoid the escaped examination,and diagnose and treat the diseases in a timely manner,which plays a significant role in improving the cure rate of the patients.

关 键 词：血流感染 血培养 需氧菌 厌氧菌 

分 类 号：R446[医药卫生—诊断学]