转Bt cry1Ah/2mG2-epsps双价基因抗虫/耐除草剂玉米研究  

Insect-Resistant and Glyphosate-Tolerant Transgenic Maize Transformed by Bt cry1Ah and 2mG2-epsps Genes

作　　者：赫福霞[1,2,3] 郎志宏[2] 张杰[4] 陆伟[2] 何康来[4] 黄大昉[2] 

机构地区：[1]东北农业大学生命科学学院,黑龙江哈尔滨150030 [2]中国农业科学院生物技术研究所,北京100081 [3]黑龙江省农业科学院生物技术研究所,黑龙江哈尔滨150086 [4]中国农业科学院植物保护研究所,北京100094

出　　处：《作物杂志》2012年第6期29-33,I0001,共6页Crops

基　　金：转基因生物新品种培育重大专项(2011ZX08003-001);国家自然科学基金资助项目(30970231);东北农业大学博士启动基金(2009RC41);黑龙江省教育厅科学技术研究项目(12521027)

摘　　要：构建了含有Bt杀虫基因cry1Ah和耐草甘膦基因2mG2-epsps的双价植物表达载体pMAGUHM,通过基因枪轰击Q31×Z31杂交组合的胚性愈伤组织,以2mG2-epsps为筛选标记,通过1mmol/L草甘膦异丙胺盐筛选,获得T0代转化植株80株,其中PCR检测有36株为阳性植株,阳性率达45%。对得到的阳性植株的T1、T2代进行了跟踪检测,证明外源基因已整合到玉米基因组中并正确表达。草甘膦及玉米螟抗性检测结果表明,有5个转基因品系表现出具有耐草甘膦和抗虫的双重特性。A bivalent plant expression vector pMAGUHM expressing the modified Bt crylAb gene and glyphosate- tolerant 2mG2-epsps gene was constructed and transformed into Zea mays L. calli Q31 × Z31 hybrid by microprejec- tile bombardment. Using the 2mG2-epsps gene as a selective marker, eighty glyphosate-tolerant maize plantlets were selected by l mmol/L glyphosate- isopropylammonium, and thirty-six glyphosate-tolerant maize plantlets were con- firmed transgenic plants by PCR analysis. The positive rate was 45%. Further analysis was performed on the T1 and T2 generation and it was confirmed that Bt crylAh gene and 2mG2-epsps gene had been integrated into the maize genome and expressed successfully. The bioassay of corn borer （Pyrausta nubilalis Hubern） resistance and glypho- sate tolerance indicated that five transgenic lines showed both glyphosate tolerant and insect resistant traits.

关 键 词：双价基因 玉米螟 耐除草剂 草甘膦异丙胺盐 抗虫 植物表达载体 BT杀虫基因 胚性愈伤组织 

分 类 号：S513[农业科学—作物学]