溴氰菊酯对γ-GCS活力和谷胱甘肽含量影响  

Effect of deltamethrin on gamma-glutamylcysteine synthetase activity and reduced glutathione content

作　　者：李煌元[1,2] 吴思英[3] 石年[1] 

机构地区：[1]福建医科大学公共卫生学院环境与健康研究所职业与环境卫生学系,福建福州350004 [2]华中科技大学同济医学院卫生毒理学系教育部环境与健康重点实验室 [3]福建医科大学公共卫生学院环境与健康研究所流行病与卫生统计学系

出　　处：《中国公共卫生》2012年第12期1587-1589,共3页Chinese Journal of Public Health

基　　金：国家自然科学基金(30371225;30800936)

摘　　要：目的探讨溴氰菊酯(DM)对PC12细胞γ-谷氨酰半胱氨酸合成酶(γ-GCS)活力和谷胱甘肽(GSH)含量的影响。方法用0、1、10、100μmol/L DM处理PC12细胞6或12 h后用四甲基偶氮噻唑蓝(MTT)法检测细胞毒性;用0、10、100μmol/L DM处理PC12细胞6或12 h后,超速离心分离细胞胞质碎片,邻苯二甲醛(OPA)柱前衍生高效液相色谱(HPLC)荧光法检测组织中γ-GCS酶活力和GSH含量。结果 100μmol/L DM处理细胞6、12 h的细胞存活率为(0.862±0.053)、(0.746±0.101),均低于对照组,差异均有统计学意义(P<0.01或P<0.001);与对照组γ-GCS酶活力(1.78±0.26)pmol/106细胞.min比较,10、100μmol/L DM处理细胞6 h组γ-GCS酶活力为(1.66±0.20)、(1.73±0.09)pmol/106细胞.min,差异均无统计学意义;与对照组GSH含量(10.18±0.23)pmol/106细胞比较,10、100μmol/L DM处理细胞6 h GSH含量为(10.19±1.18)、(10.26±0.29)pmol/106细胞,差异均无统计学意义;与对照组γ-GCS酶活力(1.94±0.19)pmol/106细胞.min和GSH含量(9.57±0.39)pmol/106细胞比较,10μmol/L DM处理细胞12 h组γ-GCS酶活力(1.73±0.21)pmol/106细胞.min和GSH含量(9.72±0.41)pmol/106细胞差异均无统计学意义,100μmol/L DM处理细胞12 h组γ-GCS酶活力(1.62±0.09)pmol/106细胞.min差异有统计学意义(P=0.006)。结论较高浓度的DM处理细胞较长时间才抑制γ-GCS酶活力,可能与DM的细胞毒性作用有关。Objective To explore the effect of deltamethrin（DM） on gamma-glutamylcysteine synthetase（γ-GCS） activity and reduced glutathione（GSH） content in PC12 cells.Methods PC12 cells were treated with 0,1,10,100 μmol/L DM for 6 hours or 12 hours,respectively.Cytotoxicity of DM on PC12 cells was measured by methyl thiazolyl tetrazolium（MTT） assay.The cytosolic fraction of PC12 cells was used to determine reduced glutathione（GSH） content and γ-GCS activity by reversed-phase high performance liquid chromatographic assay with o-phthalaldehyde pre-column derivatization.Results The survival rate of PC12 cells treated with 100 μmol DM for 6 hours or 12 hours was lower than that of control group（P0.01,P0.001）.In PC12 cells treated for 6 hours,there was no effect on γ-GCS activity and GSH content.In PC12 cells treated for 12 hours,there was no difference in γ-GCS activity and GSH content between low concentration and control group.The γ-GCS activity of the high concentration group was significantly lower than that in the control group（P0.05）.Conclusion These findings demonstrate that γ-GCS activity in PC12 cells could be inhibited by high concentration of DM treatment for a long time.

关 键 词：溴氰菊酯(DM) γ-谷氨酰半胱氨酸合成酶(γ-GCS) 谷胱甘肽(GSH) 邻苯二甲醛柱前衍生高效液相色谱荧光法 

分 类 号：R994[医药卫生—毒理学]