广州水体中人类杯状病毒污染状况检测分析  

Detection of human calicivirus in different water samples from Guangzhou city

作　　者：曾爱华[1] 金小宝[2] 朱家勇[2] 

机构地区：[1]广东药学院药科学院,广东广州510006 [2]广东药学院基础学院广东省生物活性药物研究重点实验室

出　　处：《中国公共卫生》2012年第12期1641-1643,共3页Chinese Journal of Public Health

基　　金：广东省科技计划资助项目(2006B36008001)

摘　　要：目的调查研究广东省广州地区水体中人类杯状病毒(HuCV)的污染情况。方法收集广州地区268份自来水、715份矿泉水、119份生活污水和53份池塘水,用荧光定量PCR方法检测样本中的HuCV及GⅠ、GⅡ型的诺瓦克样病毒(NLVs)和扎幌样病毒(SLVs)。结果生活污水、池塘水、自来水、矿泉水中HuCV总检出率分别为9.24%、11.32%、0、0;生活污水中的GⅠ、GⅡ型的NLVs和SLVs检出率分别为2.52%、7.56%和0;池塘水中的GⅠ、GⅡ型的NLVs和SLVs检出率分别为1.89%、9.43%、0、1.89%;在1例生活污水中检测出GⅠ、GⅡ型NLVs,1例池塘水里同时检测出GⅡ型NLVs、SLVs。结论广州地区生活用水是安全的,但环境水体中存在着HuCV不同基因型的混合污染。Objective To assess the prevalence of human calicivirus（HuCV） contamination in different water samples from Guangzhou city.Methods Totally 268 tap water,715 mineral water,119 living sewage,and 53 pool water samples were collected.All the samples were detected with real-time fluorogenic quantitative PCR for HuCV（including GⅠ,GⅡ Norwalk-like viruse,and Sapora-like viruses）.Results The positive rate of HuCV for the samples was 9.24%,11.32%,0,and 0,respectively;the positive rate of GⅠ NLVs,GⅡ NLVs,GⅠ SLVs,and GⅡ SLVs for domestic sewage samples was 2.52%,7.56%,0 and 0;the positive rate of GⅠ/GⅡ NLVs and GⅠ/GⅡ SLVs for pool water samples was 1.89%,9.43%,0,and 1.89%,respectively.GⅠ NLVs and GⅡ NLVs were detected in one domestic sewage sample;GⅡ NLVs and SLVs contamination were detected in one pool water sample.Conclusion The drinking water in Guangzhou area is safe,but different genotypes of HuCV contamination exists in other water samples.

关 键 词：人类杯状病毒(HuCV) 基因型 污染 

分 类 号：R123.1[医药卫生—环境卫生学]