盐胁迫对濒危植物长叶红砂抗氧化系统的影响  

Responses of Antioxidant System of an Endemic Halophyte Reaumuria trigyn to NaCl Stress

作　　者：薛焱[1,2] 王迎春[1] 王同智[1] 

机构地区：[1]内蒙古大学生命科学学院,内蒙古呼和浩特010021 [2]包头医学院,内蒙古包头014060

出　　处：《中国沙漠》2012年第6期1669-1673,共5页Journal of Desert Research

基　　金：国家自然科学基金项目(30720056);内蒙古自然科学基金项目(200711020501;2011BS0501);内蒙古高等学校科学研究项目(NJ09164)共同资助

摘　　要：以长叶红砂(Reaumuria trigyna)幼苗为研究对象,测定了不同浓度NaCl溶液胁迫30d的长叶红砂叶片抗氧化特征参数和自由基及膜脂过氧化指标含量,以探讨长叶红砂抗氧化系统对盐胁迫的适应机制。结果显示,高盐胁迫下长叶红砂叶片O2-的产生速率显著上升,丙二醛(MDA)含量和相对电导率都在低盐浓度下降低,高盐浓度下随着盐浓度的增加而增加;长叶红砂叶片超氧化物歧化酶(SOD)和过氧化物酶(POD)活性随盐胁迫加剧而显著提高,过氧化氢酶(CAT)的活性变化较小;随盐浓度的提高谷胱甘肽的基本含量显著提高,抗坏血酸盐的含量在降低。研究表明,长叶红砂叶片中SOD、POD和谷胱甘肽在防御盐胁迫引起的氧化性损害中起到了显著保护作用。Reaumuria trigyna were cultivated in nutrient solutions at five NaCl levels（0,100,200,300,400 mmol·L-1）for the investigation of plant antioxidant activities and active oxygen metabolism.Results show O-2 production rate significantly increases with NaCl concentration increase,and MDA and the relative electrical conductivity decrease first and then increase as NaCl concentration increases;the activities of SOD and POD significantly increase and CAT activity changes little as NaCl concentration increases;with increase of NaCl concentration,glutathione significantly increases,and ascorbic acid salt content decreases.The research revealed that SOD,CAT and glutathione are important components of antioxidant defense system for salt tolerance of R.trigyna.

关 键 词：长叶红砂 盐胁迫 抗氧化系统 

分 类 号：Q945.78[生物学—植物学]