重楼总皂苷对人胃癌MNK-45和MGC80-3细胞增殖的影响  

Effects of Rhizoma Paridis Total Saponins on Growth of Gastric Carcinoma Cell Lines MNK-45 and MGC80-3

作　　者：保永亮[1] 龚晓燕[1] 方海雁[2] 黄金玲[3] 王桐生[3] 刘先华[4] 王训翠[4] 

机构地区：[1]安徽中医学院研究生部,安徽合肥230038 [2]安徽中医学院护理学院,安徽合肥230038 [3]安徽中医学院中西医结合临床学院,安徽合肥230038 [4]安徽中医学院科研实验中心,安徽合肥230038

出　　处：《安徽中医学院学报》2012年第6期51-55,共5页Journal of Anhui Traditional Chinese Medical College

基　　金：国家自然科学基金项目(81173171)

摘　　要：目的观察重楼总皂苷(Rhizoma paridis total saponins,RPTS)对人胃癌MNK-45和MGC80-3细胞增殖的影响。方法设定5个不同浓度(5、10、20、40、80μg/ml)RPTS处理两种细胞24、48、72h后,采用四甲基偶氮唑蓝(methyl thiazolyl tetrazolium,MTT)法测定MNK-45细胞和MGC80-3细胞增殖抑制率;设定浓度为10μg/ml处理两种细胞24h,采用吖啶橙/溴化乙锭(acridine orange/ethidium bromide,AO/EB)双染观察细胞形态学变化;设定浓度为10、20μg/ml处理两种细胞6h,用流式细胞仪测定细胞周期。结果 5～80μg/mlRPTS对MNK-45和MGC80-3细胞增殖均具有显著的抑制作用(P<0.05,或P<0.01),且药物剂量越大、作用时间越长,其抑制作用越强(P<0.01);在相同药物浓度及相同作用时间下,RPTS对MNN-45细胞增殖的抑制作用较对MGC80-3作用显著增强(P<0.01);RPTS作用于两种细胞后荧光显微镜下观察均显示细胞凋亡特征;RPTS使MNK-45细胞增殖被阻滞于G0/G1期,使MGC80-3细胞增殖被阻滞于S期。结论 RPTS能抑制人胃癌MNK-45和MGC80-3细胞增殖,诱导两种肿瘤细胞凋亡,影响两种肿瘤细胞周期时相的分布,但对两种肿瘤细胞株的抑制作用和对细胞周期时相的影响存在差异。Objective To observe the effects of Rhizoma Paridis total saponins （RPTS） on the growth of human gastric carcinoma cell lines MNK-45 and MGC80 3. Methods MNK 45 and MGC80 3 cells were treated with 5, 10, 20, 40, and 80 μg/ml RPTS for 24, 48, and 72 h, and then methyl thiazolyl tetrazolium （MTT） assay was used to measure the growth inhibition rates of the two cells. MNK 45 and MGC80 3 cells were treated with 10 μg/ml RPTS for 24 h, and then acridine orange/ethidium （AO/EB） staining was used to observe the morphological changes of the two cells. MNK-45 and MGC80 3 cells were treated with 10 and 20 μg/ml RPTS, and then flow cytometry was used to determine the changes in cell cycles of the two cells. Results RPTS （5-80 μg/ml） had sig nificant inhibitory effects on the growth of MNK-45 and MGC80 3 cells （P〈0.05 or P〈0.01）, and the higher the dosage was, the longer and stronger the inhibitory effect was （P〈0.01）. RPTS had significantly stronger inhibitory effects on MNN 45 cells than on MGC80-3 cells when applied at the same concentration for the same period （P〈0.01）. Both ceils showed apoptotic features under a fluorescence microscope after being treated with RPTS. Treated with RPTS, MNK-45 cells were arrested in G0/G1 phase, and MGC80 3 cells in S phase. Conclusion RPTS can inhibit cell growth, induce apoptosis, and affect cell cycle in human g~.stric carcinoma cell lines MNK-45 and MGC80-3, but the effects on cell growth and cell cycle were different in them.

关 键 词：重楼总皂苷 人胃癌细胞株 增殖抑制作用 细胞周期 细胞凋亡 

分 类 号：R285.5[医药卫生—中药学]