氢溴酸右美沙芬咀嚼片在正常人体内的生物利用度  

Boavailability of dextromethorphan hydrobromide chewing tablet

作　　者：吴琳华[1] 樊宏伟[1] 付红炎 田辉凯[1] 孙考祥[1] 

机构地区：[1]哈尔滨医科大学附属二院临床药学药物研究所,黑龙江哈尔滨150086

出　　处：《中国药学杂志》2000年第6期404-406,共3页Chinese Pharmaceutical Journal

摘　　要：目的　建立反相高效液相色谱法测定人血浆中氢溴酸右美沙芬的浓度。方法　采用Nova PakC18色谱柱 ,以乙腈 水 36 %乙酸 (5 0∶49∶1) ,用三乙胺调 pH至 4.3,流速 :1ml·min-1,荧光检测 ,激发波长 2 80nm ,发射波长 310nm。结果　氢溴酸右美沙芬约 1.95min达高峰浓度 ,cmax为 (6 .2 0± 1.32 )ng·ml-1。结论　本法简便、灵敏、专属性好 。OBJECTIVE To develop a RP HPLC assay for the determination of dextromethorphan hydrobromide (DM) in healthy volunteers.METHODS The analytical colunm was packed with Nova Pak C 18 .The mobile phase consisted of a mixture of acetonitrile water acetic acid (50∶49∶1).Triethylamine was used to adjust pH to 4.3.Flow rate was 1 ml·min -1 .Fluor escence detector was used with Ex at 280 nm and Em at 310 nm.RESULTS: T max ws about 1.95 min. c max was (6.20±1.32) ng·ml -1 .CONCLUSION:The assay here was sensitive,simple and accurate,can be used to study pharmacokinetics of DM.

关 键 词：氢溴酸右美沙芬 高效液相色谱法 相对生物利用度 

分 类 号：R974.2[医药卫生—药品] R969.1[医药卫生—药学]