高效液相色谱柱切换法测定犬血浆中茶碱  

Determination of theophylline in dog plasma by high performance liquid chromatography with column switching

作　　者：叶利民[1] 李章万[1] 郭平[1] 洪诤[1] 

机构地区：[1]华西医科大学药学院,四川成都610041

出　　处：《中国药学杂志》2000年第6期407-409,共3页Chinese Pharmaceutical Journal

摘　　要：目的　建立茶碱犬血浆血药浓度HPLC柱切换测定方法。方法　LichropredRP18(5 0mm× 4mm ,2 5～ 40 μm )预处理柱 ,水为预处理流动相。Shim packCLC -ODS(15 0mm× 6mm ,5 μm)分析柱 ,甲醇 水 (2 2∶78)为分析流动相。紫外检测波长2 70nm。结果　茶碱血浆最低检测浓度为 0 .0 1μg·ml-1,线性范围 1～ 32 μg·ml-1,平均净化回收率为 (95 .8± 1.33) %。结论　用于测定 12只中国健康杂种犬灌服茶碱缓释片剂 2 0 0mg后血药浓度 。OBJECTIVE High performance liquid chromatography with column switching was developed for the determination of theophylline in plasma of dog.METHODS The plasma samples were injected onto a pretreatment column packed with Lichromprep the RP 18(25～40 μm)after simple dilution.Distilled water was used to wash out protein and other impurities in plasma.After switching,the concentrated theophylline was eluted in the back flush mode onto a Shimpack CLC ODS column with methanol water (22∶78)as mobile phase.Purge solutions were used for clean up and for regenerating the pretreatment column.RESULTS The limit of detection was 0.001 mg·L -1 plasma.The linear range was 1～32 mg·L -1 .The RSD of intra and inter day were less than 3.37% and 5.36% for theophylline in dog plasma,respectively.CONCLUSION The method was successfully used to determine the pharmacokinetics of theophylline sustained release tablets in 12 dogs.

关 键 词：茶碱 柱切换 血药浓度 高效液相色谱法 

分 类 号：R974.3[医药卫生—药品] R969.1[医药卫生—药学]