微波协助提取在中药饮片含量测定中的应用(6)——微波法与药典法测定野菊花中蒙花苷含量比较  

Determination of Microwave-Assisted Extraction of Chinese Cut Crude Drugs(6) Comparison of the Determination of Linarin from Chrysanthemum indicum Between Microwave-Assisted Extraction and Chinese Pharmacopeia Method

作　　者：方婧[1] 杨洪军[1] 付梅红[1] 安琪[2] 张贝贝[1] 王祝举[1] 杨岚[1] 张东[1] 

机构地区：[1]中国中医科学院中药研究所,北京100700 [2]首都医科大学,北京100069

出　　处：《中国实验方剂学杂志》2012年第24期94-96,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金："重大新药创制"科技重大专项(2009ZX09308-003);"国家科技支撑计划子课题"(2006BAI09B07-02)

摘　　要：目的:建立微波协助提取法提取野菊花中有效成分蒙花苷含量测定方法,比较微波协助提取法与药典法提取蒙花苷优势。方法:采用Dikma Diamonsil C18色谱柱(4.6 mm×150 mm,5μm),流动相甲醇-水-冰醋酸(26∶23∶1),检测波长334nm,柱温30℃,流速1 mL.min-1。结果:微波提取时间15 min,提取温度100℃,提取溶剂50%乙醇。蒙花苷在0.04～1.2μg呈良好线性关系(r=0.999 9),平均回收率100.13%(n=6)。结论:微波提取法较药典法提取蒙花苷更简便快速、准确,为一种符合环保绿色理念的含量测定方法,可用于检测野菊花饮片中蒙花苷含量。Objective： To establish a microwave-assisted extraction method for the extraction of the linarin from Chrysanthemun indicum. Comparison of the determination of linarin from C. indicum between microwave-assisted extraction and Chinese pharmacopeia method was made. Method： Dikma Diamonsil Cl8 column （4.6 mm × 150 mm, 5 μm） was used. The mobile phase cousisted of methanol-water-acetic acid （26： 23：1 ）. The temperature of column was kept at 30 ℃. The flow rate was 1.0 mL ·min^-1 and the detection wavelength was at 334 rim. Result： Microwave extractive time was 15 min, the temperature was 100 ℃ , the extraction solvent was 50% ethanol. The calibration curve was in good linearity within the range from 0.04 to 1.2 μg （ r = 0. 999 9）. The average recovery was 100. 13% （n =6）. Conclusion： The method is simple, rapid, as well as precise and reliable, and can be used for the determination of linarin from C. indicum.

关 键 词：微波提取 野菊花 蒙花苷 含量测定 

分 类 号：R284.1[医药卫生—中药学]