HPLC测定湿疹纳米乳喷雾剂中丹皮酚的含量  

Determination of Paeonol Concentration in Eczema Nanoemulsion Spray by HPLC

作　　者：陈光宇[1] 毛骁丽[1] 何群[2] 赵碧清[1] 郭建生[1] 

机构地区：[1]湖南中医药大学药学院,长沙410208 [2]湖南中医药大学中药药剂学重点学科,长沙410208

出　　处：《中国实验方剂学杂志》2012年第24期97-100,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家科技部"十一五"支撑计划(2008BAI53B042)

摘　　要：目的:建立湿疹纳米乳喷雾剂中丹皮酚的HPLC定量方法,控制该制剂的质量。方法:采用高效液相色谱法,Phenomenex C18-ODS色谱柱,流动相甲醇-水(55∶45),流速1.0 mL.min-1,检测波长274 nm。结果:标准曲线线性范围在10.0～100.0 mg.L-1(r=0.999 9),48 h内稳定,加样回收率99.60%,RSD 1.123%。理论塔板数均在6 000以上。分离度达1.5以上。结论:方法简便、快速、灵敏、准确,稳定性及重复性好,可作为该制剂中丹皮酚含量测定的常规方法。Objective： To establish a method for the determination of paeonol in Eczema Nanoemulsion spray by HPLC. Method： Using HPLC technique, we detected the samples by the C18 ODS column of Phenomenex company under the wave length at 274 nm, the chromatographic conditions were phenomenex C18-ODS column, mobile phase of methanol-water （55：45） , flow rate of 1.0 mL ·min^-1 and detection wave-length at 274 nm. Result： The standard curve was linear over the range of 10.0-100.0 mg ·L^-1, with the correlation coefficient of 0. 999 9. It is steady in 48 hour. The average recovery was 99.70% with RSD 1.12% （n = 9）. The theoretical plates are above 6 000, separating degree is greater than 1.5. Conclusion： The analytical method is simple, rapid, sensitive, accurate; stability and reproducibility are good, and the method could be used to determine content of paeonol in a Chinese medicine Eczema Nanoemulsion spray.

关 键 词：湿疹纳米乳喷雾剂 高效液相色谱 丹皮酚 

分 类 号：R284.1[医药卫生—中药学]