北细辛不同提取物中总马兜铃酸的含量测定  

Determination of Total Aristolochic Acid in Different Extracts of Asarum heterotropoides

作　　者：于艳[1] 修丽丽[2] 魏新智[1] 孙科峰[1] 

机构地区：[1]辽宁中医药大学附属医院,沈阳110032 [2]辽宁中医药大学,辽宁大连116600

出　　处：《中国实验方剂学杂志》2012年第24期141-143,共3页Chinese Journal of Experimental Traditional Medical Formulae

摘　　要：目的:测定北细辛不同提取物中总马兜铃酸的含量,为其临床应用提供实验依据。方法:以水、乙酸乙酯、30%乙醇、50%乙醇、70%乙醇、95%乙醇提取北细辛药材,以马兜铃酸A为对照,采用紫外分光光度法,检测波长为390 nm。结果:马兜铃酸A含量在3.0～30.0 mg.L-1与吸收度呈良好线性关系,Y=19.844X+0.027 2(r=0.999 6),平均回收率为98.51%,RSD 1.32%。北细辛不同提取物中总马兜铃酸的含量为1.34%～3.15%,以水提取物含量最低,95%乙醇提取物含量最高。结论:方法测定结果准确可靠,重复性好,可用于北细辛提取物中总马兜铃酸的含量测定。Objective： To determine the content of total aristolochic acid in different extracts of Asarum heterotropoides, and provide the basis for clinical application of A. heterotropoides. Method： A. heterotropoides was extracted with water, ethyl acetate, 30% ethanol, 50% ethanol, 70% ethanol and 95% ethanol as extracting solvent. The ultraviolet spectrophotometry was used with aristolochic acid A as the chemical reference. The UV detector wavelength was set at 390 nm. Result： The content of aristolochic acid A showed a linear relation with the spectrophotometric absorbance within the range of 3.0-30.0 mg · L^-1, and the linear equation was Y = 19. 844X ＋ 0. 027 2 （r = 0. 999 6）. The average recovery was 98.51% , and RSD was 1.32%. The total aristolochic acid content varied from 1.34%-3.15%. It was the lowest in the water extract and highest in the 95% ethanolic extract. Conclusion： The method is reliable, accurate and suitable for determining the content of the total aristolochic acid in extracts of A. heterotropoides.

关 键 词：北细辛 提取物 总马兜铃酸 紫外分光光度法 

分 类 号：R284.1[医药卫生—中药学]