秦岭柴胡不同提取物保肝作用量效关系研究  

Experimental Study on Dose-effect Relationship of Hepatoprotective Effect of the Different Extraction from Bupleurum longicaule var.giraldii

作　　者：卫昊[1] 刘清[1] 卫伟光[2] 

机构地区：[1]陕西中医学院,陕西咸阳712046 [2]陕西省西安植物园,西安710061

出　　处：《中国实验方剂学杂志》2012年第24期237-240,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：陕西省教育厅科学研究计划项目(2010JK499);咸阳市科技发展计划项目(2010-K14-02(5))

摘　　要：目的:探讨两种产地秦岭柴胡不同提取物对D-氨基半乳糖致急性肝损伤小鼠的保护作用,并初步筛选最佳提取工艺和活性部位。方法:将小鼠分为9组:空白对照组,半乳糖胺模型组,联苯双酯滴丸(150 mg.kg-1),秦岭柴胡1号样品水提组、65%醇提组、95%醇提组,秦岭柴胡2号样品水提组、65%醇提组、95%醇提组。剂量为5.0 g.kg-1,灌胃给药,给药体积20 mL.kg-1,模型组和空白组分别灌胃给予同体积生理盐水,每天1次,连续给药7 d后,采取小鼠腹腔注射D-氨基半乳糖盐酸盐溶液造模,16 h后眼静脉采血,测定血清中谷丙转氨酶(ALT)、谷草转氨酶(AST)活性;同时测定肝匀浆中丙二醛(MDA)含量和超氧化物歧化酶(SOD)活性。结果:2种产地秦岭柴胡的水提组、65%醇提组、95%醇提组均能不同程度降低CCl4模型小鼠血清ALT,AST活性,降低肝脏MDA的含量,增强SOD活性,其中以东太白产秦岭柴胡65%醇提物作用最强(P<0.01,P<0.05)。结论:秦岭柴胡对D-氨基半乳糖盐酸盐所致小鼠急性肝损伤具有较明显的保护作用,其保肝作用与柴胡皂苷a,d含量存在一定的量效关系。Objective： To discuss protective effect of different extractions of two origin Bupleurum Iongicaule var. giraldii on acute liver injury in mice caused by intraperitoneal injection （ip） D-galactosamine, and to screen the optimum process and the active site of extraction. Method： The mice were divided into 9 groups： control group, model group, galactosamine group, bifendate group （150 mg ·kg^-1）, water extraction group, 65% ethanol extract group, 95% ethanol extract group of B. longicaule var. giraldii sample No. 1 and No. 2, delivery 20 mL·kg^-1 by intragastric administration （ig）. Model group and control group were given normal saline by ig once a day with the same dose. After continuous administration of 7 days, model of mice were made by intraperitoneal injection of D-galactosamine hydrochloride solution, and after 16 hours, ocular venous blood were collected to determine the serum alanine aminotransferase （ALT） and aspartate aminotransferase （AST） activities and the malondialdehyde （MDA） content and superoxide dismutase （SOD） activity in liver homogenate. Result： Two origin of B. longicaule var. giraldii on aqueous extraction group, 65% ethanol group and 95% ethanol group showed that they all could decrease the serum ALT and AST activity in model, reduce the content of liver MDA and increase the SOD activity, and the 65% ethanol extract of B. longicaule var. giraldii gathered from East Taibai Mountain had the strongest effect （P 〈 O. O1, P 〈 0. 05）. Conclusion： B. longicaule vat. giraldii has obvious protective effect on D-galactosamine hydrochloride induced acute liver injury in mice. There is a dose-effect relationship between its hepatoprotective effect and saikosaponin a, d content.

关 键 词：秦岭柴胡 D-氨基半乳糖盐酸盐 保肝作用 

分 类 号：R285.5[医药卫生—中药学]