4个新黄酮类化合物抗中性粒细胞呼吸爆发作用比较  

Comparison of Activities of Four Neoflavonoids on Neutrophils Respiratory Burst

作　　者：邵峰[1] 唐芳瑞[1] 陈慧娟[1] 王倩[1] 刘荣华[1] 任刚[1] 陈兰英[2] 黄慧莲[1] 

机构地区：[1]江西中医学院现代中药制剂教育部重点实验室,南昌330004 [2]中药固体制剂制造技术国家工程研究中心,南昌330006

出　　处：《中国实验方剂学杂志》2012年第24期280-282,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(81060373);江西省自然科学基金项目(2008GDY0019);江西省卫生厅基金项目(2009A100);江西省研究生创新专项资金项目(YC10A123)

摘　　要：目的:比较4个新黄酮类化合物抗中性粒细胞呼吸爆发作用。方法:采用化学发光法,以抗坏血酸(VC)为参照,以IC50作为评价指标,比较4个新黄酮类化合物抗中性粒细胞呼吸爆发作用的强弱关系。结果:4个新黄酮类化合物抗中性粒细胞呼吸爆发作用呈对数量效关系,且作用强弱依次为2,4-dihydroxy-5-methoxy-benzophenone,R(+)-dalbergiphenol,R(-)-latifolin,2-O-methyllatifolin。结论:新黄酮类化合物分子结构中交叉共轭结构,有利于化合物抗中性粒细胞呼吸爆发能力的提高。A环酚羟基是重要的活性基团,而B环酚羟基和甲氧基的取代可减弱化合物的活性。Objective： To compare inhibition effect of four neoflavonoids on respiratory burst of rat neutrophils. Method： Vc as reference, chemi-luminescence was used to compare strengths of the four neoflavonoids compounds in antineutrophill rspiratory burst. Result： Four neoflavonoids have different effect on neutrophils respiratory burst, and the marked logarithmic relationship existed between bioactivity and chemical structure. The inhibition abilities of four neoflavonoids on neutrophils respiratory burst were as follows： 2, 4- dihydroxy-5-methoxy-benzophenone, R （ ＋ ） -dalbergiphenol, R （-） -latifolin, 2-O-methyllatifolin. Conclusion ： In chemical structure of neoflavonoids, the Cross-Shaped conjugation of A ring was favorable for the inhibition effect of four neoflavonoids on neutrophils respiratory burst, and the phenolic hydroxyl group, in A ring, was the key bioactivity group. In addition, the hydroxymethylation of hydroxyl in B ring was not favorable for improving bioactivity.

关 键 词：新黄酮 中性粒细胞呼吸爆发 化学发光法 

分 类 号：R285.5[医药卫生—中药学]