蛇葡萄素诱导人胃癌细胞凋亡的研究  

Study of Ampelopsin on Apoptosis in Human Gastric Cancer Cells

作　　者：郑作文[1] 吕林艳[1] 王小琴[1] 陶林[1] 

机构地区：[1]广西中医药大学药学院,南宁530001

出　　处：《中国实验方剂学杂志》2012年第24期292-294,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：广西中医药大学校级重点课题(ZD2010006)

摘　　要：目的:研究蛇葡萄素对人胃癌细胞(SGC-7901)凋亡的影响。方法:MTT比色法测定细胞的增殖抑制率,流式细胞仪Annexin-V FITC/PI双染检测蛇葡萄素33.3,50,75 mg.L-1作用下SGC-7901细胞的凋亡及各细胞周期。结果:蛇葡萄素作用于SGC-7901 48 h后,可明显促进细胞早期凋亡,50,75 mg.L-1细胞凋亡率为(29.03±6.21)%,(43.43±1.00)%,明显高于空白组(11.83±4.67)%(P<0.05,P<0.01)且呈剂量依赖关系。蛇葡萄素33.3,50,75 mg.L-1作用于SGC-7901细胞48 h后,G1期细胞分别为(52.07±4.41)%,(57.07±2.70)%,(58.43±2.66)%明显高于空白对照组(42.00±3.04)%(P<0.05,P<0.01,P<0.01)。结论:蛇葡萄素能诱导SGC-7901细胞凋亡并使细胞周期阻滞在G1期。Objective： To study ampelopsin on apoptosis in human gastric cancer cells （SGC-7901）. Method： Cell proliferation inhibition rate was measured by MTT, The situation in different apoptosis phase was evaluated by flow cytometry （Annexin-V/PI double staining）. Result： Ampelopsin could significantly promote early apoptosis in SGC-7901 after 48h. Ampelopsin of 50 and 75 mg · L^-1 induced apoptosis of （29.03 ± 6.21 ）% , and （43.43 ± 1.00）% with a dose-dependent relationship, significantly higher than that in the control group （11.83 ±4.67）% （P 〈0.05, P 〈0.01, P 〈0.01）. Cells in G1 phase was （52.07 ±4.41）%, （57.07 ± 2.70） %, （58.43 ± 2.66） %, significantly higher than （42.00 ± 3.04） % in control group （ P 〈 0.05, P 〈0.01, P 〈0.01）. Conclusion： AmlJelopsin could induce the SGC-7901cell apoptosis and block the cells in G1 phase.

关 键 词：蛇葡萄素 人胃癌细胞 细胞凋亡 

分 类 号：R285.5[医药卫生—中药学]