诺沃克病毒实时荧光RT-PCR检测方法的建立  

Development of a Real-time RT-PCR for Detection of Norwalk Virus

作　　者：邱杨[1] 赵丽[1] 卢小雨[2] 陈进会[1] 兰文生[2] 刘建丽[1] 王伟[1] 

机构地区：[1]东莞出入境检验检疫局,广东东莞523072 [2]深圳出入境检验检疫局,广东深圳518045

出　　处：《中国动物检疫》2012年第12期29-31,共3页China Animal Health Inspection

基　　金：质检公益性科技计划项目(201110036)

摘　　要：本文建立了一种用于诺沃克病毒实时荧光RT-PCR检测的引物和方法。分析已报道的诺沃克病毒基因组序列,分别设计引物和荧光探针。通过实时荧光RT-PCR检测,得到诺沃克的特异性荧光曲线,检测结果准确、可靠。In this paper, a real-time RT-PCR was developed for detection of Norwalk virus, with the primers and fluorescence probe designed according to the reported genome sequence of Norwalk virus. By the Real-time RT-PCR detection, a specific fluorescence curve was got. It was shown that the test results were exacted and reliable.

关 键 词：诺沃克病毒 引物 RT-PCR 

分 类 号：S941.41[农业科学—水产养殖]