基于SRAP标记的刺葡萄亲缘关系分析  

Relationship analysis for Vitis davidii Foёx with SRAP molecular markers

作　　者：刘昆玉[1,2] 徐丰[1,2] 石雪晖[1,2] 钟晓红[1,2] 杨国顺[1,2] 倪建军[1,2] 

机构地区：[1]湖南农业大学园艺园林学院,湖南长沙410128 [2]湖南省葡萄工程技术研究中心,湖南长沙410128

出　　处：《湖南农业大学学报（自然科学版）》2012年第6期607-611,共5页Journal of Hunan Agricultural University(Natural Sciences)

基　　金：国家农业部农业公益性行业科研项目(nyhy2x07–027);国家农业产业技术体系建设专项(nycytx–30–zt–06);湖南省科学技术厅重点科研项目(2009CK-2010);湖南省教育厅省级科技计划(成果推广计划)项目(09CY008)

摘　　要：运用SRAP标记对分布在湖南省内的21份刺葡萄资源进行遗传多样性与亲缘关系分析。结果表明:从84对SRAP引物组合中筛选出的7个引物组合中共扩增出61条带,其中有34条稳定的多态性条带,多态性比率为55.7%;运用NTSYS软件计算出21份材料的成对遗传相似系数为0.80～0.99,在遗传相似系数0.92处可将全部材料划分为3个类群;UPGMA聚类分析表明,在湖南省境内分布的刺葡萄有6个基本类型,并推测出了农大1号至农大9号育种材料(2003年由湖南农业大学运用秋水仙素处理不同地区刺葡萄种籽得到的诱变材料,由于经过3次搬迁,现已遗失亲本档案)的亲本。Sequence-related amplified polymorphism （SRAP） molecular markers were used to detect the genetic diversity and relationship of 2l accessions of Vitis davidii Foex. From 84 pairs of primers, 7 pairs were selected out. Sixty-one clear bands were amplified using these 7 pairs of primers, of which 34 were stable morphological bands, accounting for 55.7% of total bands. Revealed by NTSYS software, the genetic similarities of 21 accessions ranged from 0.80 to 0.99. Cluster analysis showed that 21 accessions were classified into 3 cluster groups with genetic similarity of 0.92. UPGMA cluster analysis showed that there are 6 types of Vitis davidii Foex in Hunan, and we successfully deduced the suspected parents （which were lost during 3-time relocations） of Nongda No.1 to No.9 which come from different regions of Vitis davidii Foex seeds by colchicine treatment.

关 键 词：刺葡萄 亲缘关系 SRAP标记 

分 类 号：S321[农业科学—作物遗传育种]