10种铁线莲的核型特征及核型似近系数聚类分析  

Karyotype features of 10 species of Clematis and cluster analysis of karyotypies resemblance-near coefficient

作　　者：彭绿春[1,2,3,4] 于恒隽[5] 余娜 瞿素萍[1,2,3,4] 张艺萍[1,2,3,4] 解玮佳[1,2,3,4] 李世峰[1,2,3,4] 丁鲲[7] 

机构地区：[1]云南省农业科学院花卉研究所,云南昆明650205 [2]农业部花卉产品质量监督检验测试中心,云南昆明650205 [3]云南省花卉育种重点实验室,云南昆明650205 [4]云南花卉技术工程研究中心,云南昆明650205 [5]华南农业大学园艺学院,广东广州510642 [6]云南省花卉产业办公室,云南昆明650205 [7]云南省农村科技服务中心,云南昆明650021

出　　处：《湖南农业大学学报（自然科学版）》2012年第6期617-622,共6页Journal of Hunan Agricultural University(Natural Sciences)

基　　金：云南省科技计划项目(2011EB111)

摘　　要：为探明铁线莲核型特征与种间的亲缘关系,以10种(品种)铁线莲组培苗幼嫩根尖为材料,应用常规压片法分析染色体特征,并进行核型似近系数聚类分析。核型特征分析结果表明:除1个栽培种‘杰克曼’的染色体数为14外,其余9个种的染色体数2n=16,染色体基数为8;10种铁线莲核型特征参数种间存在的差异,可作为铁线莲属植物分类和亲缘关系判定的依据。以核型似近系数进行聚类分析,在核型似近系数为0.818 1时,10种铁线莲聚为3类;在核型似近系数为0.893 9时,10种铁线莲聚为4类。Taking young root tip of in vitro plantlet of 10 species of Clematis as material, chromosome feature analysis and cluster analysis of karyotype resemblance-near coefficient were conducted by using conventional pressed slice method, to discuss the karyotype features and genetic relationship between species. The results suggested that the chromosome number of ＇Jackmanii＇was 14, the chromosome number of the other species were 16, and basic number of chromosome was 8. There existed differences of karyotype parameters in 10 species of Clematis, so karyotype datas could provide a base for the classification and genetic relationship identification of Clematis. To make cluster analysis of karyotypies resemblance-near coefficient, when karyotype resemblance-near coefficient（2） was 0.818 1, 10 species of Clematis could be divided into 3 groups, when 2 was 0.893 9, 10 species of Clematis could be divided into 4 groups.

关 键 词：铁线莲 核型特征 核型似近系数 聚类分析 

分 类 号：S682[农业科学—观赏园艺]