增殖细胞核抗原在判断肝再生中的作用及奥美拉唑的促肝再生作用  

The role of PCNA in evaluating liver regeneration and the effect of omeprazole on liver regeneration

作　　者：李俊生[1] 嵇振岭[1] 严航[2] 

机构地区：[1]南京铁道医学院附属医院普外科,210009 [2]南京铁道医学院病理教研室

出　　处：《中华普通外科杂志》2000年第6期352-354,共3页Chinese Journal of General Surgery

摘　　要：目的　研究增殖细胞核抗原 (PCNA)计数在判断肝组织再生过程中的作用以及质子泵抑制剂奥美拉唑在肝部分切除术中对肝再生的促进作用。方法　取成年SD大鼠 5 0只 ,制备成肝硬化模型 ,随机分为对照组及处理组 ,全身麻醉下行 70 %肝切除术 ,术后按组给予生理盐水 1ml/kg·d及奥美拉唑 0 2mg/kg·d皮下注射共 1周。 1周后每组取 8只大鼠抽取静脉血样、取下肝脏称重 ,并制备肝组织光学显微镜、电子显微镜及PCNA染色标本。两周后 ,余下 6只大鼠同样处理。结果　在治疗 1周后 ,奥美拉唑组血清胃泌素水平为 (415± 2 2 )ng/L ,而治疗前为 (312± 7)ng/L ;而对照组治疗前后差异有显著意义 (P <0 0 5 )。在 1周后 ,奥美拉唑组的再生肝相对肝重量为 (6 4± 7) % ,对照组为(5 5± 6 ) % (P <0 0 5 )。治疗 2周后 ,奥美拉唑组的再生肝相对肝重量 (RLW)为 (6 9± 6 ) % ,对照组为(6 2± 5 ) % ,同样差异有显著意义 (P <0 0 5 )。肝细胞有丝分裂指数 (MI)在奥美拉唑组与对照组分别为 (2 0± 0 3) %及 (1 1± 0 2 ) %。PCNA阳性计数奥美拉唑组及对照组分别为 9 2 %及 4 1%(P <0 0 5 )。结论　PCNA在判断肝再生时与RLW及MI等指标一致 ,奥美拉唑可促进肝硬化肝部分切除术后肝脏的再生。Objective To investigate the role of PCNA counting (proliferative cell nuclear antigen) in evaluating the liver regeneration and whether omeprazole has an effect to enhance liver regeneration. Methods CCl 4 induced cirrhotic rats were divided into two groups, undergoing 70% hepatectomy. Group one received omeprazole 0 2?mg/kg, sc, daily; Group two, as the control, received 0 9% NS, sc, 1?ml/kg, daily. One week later 8 rats of in each group were sacrificed,venous blood was drawn and liver sent for microscope examination, and PCNA determination, the RLW (relative liver weight) was determined.Procedure was repeated 2 weeks later on 6 rats in each group. Results In the omeprazole group, the serum gastrin concentration, RLW and MI were higher than those in control group, and PCNA was in positive correlation with MI (mitotic index). Conclusions PCNA could be used to evaluate the degree of liver regeneration. Omeprazole can enhance the cirrhotic liver regeneration after hepatectomy.

关 键 词：肝再生 增殖细胞核抗原 奥美拉唑 

分 类 号：R965[医药卫生—药理学]