石栗树茎段组织培养的研究  

Research on Tissue Culture of the Stem in Aleurites moluccana

作　　者：韩佳宇[1] 伍豪[1] 欧克纬[1] 宋炫旻[1] 

机构地区：[1]广西大学农学院,南宁530004

出　　处：《中国农学通报》2012年第32期204-208,共5页Chinese Agricultural Science Bulletin

基　　金：广西科技厅资助项目"优质能源植物石栗树开发利用关键技术研究与种植示范"(桂科攻0992021-3);广西教育厅资助项目"优质石栗资源搜集及其优良种质组培快繁研究"(200809MS152)

摘　　要：建立石栗组织培养的技术体系,筛选出适合诱导愈伤组织和愈伤组织分化的培养基。从外植体消毒,激素配比,培养基筛选等方面对石栗组织培养进行了探索。结果表明诱导愈伤组织的主要因素是细胞分裂素和生长素的比值。TDZ对愈伤组织诱导丛生芽有抑制作用。诱导愈伤组织的最适培养基为:1/2MS+ZT2mg/L+NAA0.5mg/L+氯化胆碱2mg/L,诱导愈伤组织丛生芽的最适培养基为1/2MS+6-BA1mg/L+IBA0.1mg/L。影响诱导愈伤组织的主要因素是细胞分裂素和生长素的比值。The aims were to establish the technique system which was about the tissue culture of Aleurites moluccana, and then choose the media which was the most appropriate for calli induction and differentiation. The results showed that, the ratio of the cytokinin and the archusia was the primary factor that acts on the calli induction. TDZ inhibited the induction that from the calli to the axillary buds. The best media for calli induction was： 1/2MS＋ZT 2 mg/L＋NAA 0.5 mg/L＋choline chloride 2 mg/L. The most suitable media for inducing axillary buds was 1/2MS＋6-BA 1 mg/L ＋IBA 0.1 mg/L. The ratio of the cytokinin and the archusia was the imoortant factor for calli induction.

关 键 词：石栗 愈伤诱导 愈伤分化 组织培养 

分 类 号：S792.99[农业科学—林木遗传育种]