有毒甲藻细胞密度测定方法的比较研究  

Comparative study on cell counting method with toxic dinoflagellate Prorocentrum lima

作　　者：曾玲[1] 龙丽娟[1,2] 梁计林[2] 龙超[2] 

机构地区：[1]湛江师范学院化学科学与技术学院,广东湛江524048 [2]中国科学院南海海洋研究所/中国科学院海洋生物资源可持续利用重点实验室/广东省海洋药物重点实验室,广东广州510301

出　　处：《广东农业科学》2012年第21期148-150,共3页Guangdong Agricultural Sciences

基　　金：中国科学院重要方向性项目(KSCX2-YW-R-093);中国科学院知识创新工程重要方向项目(KSCX2-EW-G-12)

摘　　要：采用显微镜计数法、荧光分光光度法、叶绿素a含量测定法、可见分光光度法对利玛原甲藻(Prorocentrum lima)指数生长期不同浓度的培养液进行细胞密度测定。结果表明,后3种细胞计数方法与显微镜计数法相比较,均有较好的线性关系;同时对不同生长期的利玛原甲藻培养液进行测定,结果表明,4种方法的生长曲线一致性良好。比较分析发现,可见分光光度法适用于不同细胞密度范围的测定,操作简单、耗时短、不需要破坏样品、适于批量操作,可确定为便捷、有效、可行的利玛原甲藻细胞计数法。In this study, four cell-counting methods of microscope, Fluorescence Spectrophotography, Visible Spectrophotography and Chla Containing Measurement were used to measure the amount of the exponential phase cell of Prorocentrum lima. The insightful comparison on the interrelations of the last three mentioned cell counting methods was made with the method of microscope, the results revealed the sound linear interrelations among them. Different growth phase of P. lima were simultaneously measured, and then growth curves were drew basis on the data from different methods. The results showed good concordance between these measurement methods. Particularly, for Visible Spectrophotography Methods, the operating procedures were simple, fast, suitable for wide cell-density ranges, batch measurements and the test sample ceils were not destructed and could be reused for further research after measurement. Therefore, visible spectrophotography was convenient, high-efficiency and reliable approach for cell counting of P. lima.

关 键 词：利玛原甲藻 细胞计数法 生长曲线 

分 类 号：S968.4[农业科学—水产养殖]