香蕉枯萎病菌SNF1基因的克隆及生物信息学分析  

Cloning and bioinformatics analysis of SNF1 gene from Fusarium oxysporum f.sp.cubense

作　　者：刘一贤[1,2] 周端咏[1,2] 毛超[1,2] 汪军[1,2] 黄俊生[1] 

机构地区：[1]海南大学环境与植物保护学院,海南海口570100 [2]中国热带农业科学院环境与植物保护研究所/农业部热带农林有害生物入侵监测与控制重点开放实验室,海南儋州571737

出　　处：《广东农业科学》2012年第21期160-164,共5页Guangdong Agricultural Sciences

基　　金：国家公益性行业(农业)科研专项(200903049);中央级公益性科研院所基本科研业务费专项(2012hzs1J003-1)

摘　　要：为了解SNF1基因在香蕉枯萎病菌致病过程中的作用以及FOC1和FOC4两个生理小种之间的致病力差异关系,采用了PCR、RT-PCR的方法扩增了2个生理小种的SNF1基因,测序并采用生物信息学软件对相似序列进行搜索、比对,并对该基因编码的预测蛋白进行氨基酸序列比对和分析。研究结果显示,2个生理小种的SNF1基因的ORF分别为2 136、2 130 bp,二者相差6个碱基,同源性为99.5%。FOC1和FOC4的SNF1基因分别编码711、709个氨基酸,相差两个氨基酸,为丝氨酸(FOC1)和谷氨酰胺(FOC1)。生物信息学软件预测结果表明该蛋白N端不存在信号肽,但具有两个相同的功能结构域位点,分子量约为79 ku,pI为6.80。In order to know the roles of SNF1 gene in invasion process of Fusarium oxysporum f.sp. cubense （FOC） into banana plants, and whether it determined the difference in pathogenicity of the race 1 and 4 of FOC to the same banana cuhivar, the SNF1 genes were amplified by polymerase chain reaction and reverse transcription polymerase chain reaction, and the amplification products were then sequenced. After that, the nucleotide sequences of the SNF1 gene and the sequences of their products were aligned using DNAStar Meglignment software. The results revealed that the lengths of the open reading frames of SNF1 genes from two races of Fusarium oxysporum f.sp.cubense were 2 136 bp and 2 130 bp, and they encoded the polypeptides of 711 and 709 amino acids with 79 ku of calculated molecular weight and 6.80 of pI. The SNF1 gene from FOC race 1 showed 99.5% similarity to that of FOC race 4. Besides race 1 more than race 4 six nucleotide sequence and two amino acids sequence, they were serine and glutamine.

关 键 词：香蕉枯萎病菌 生理小种 SNF1基因 细胞壁降解酶 致病性 

分 类 号：Q789[生物学—分子生物学]