适于ISSR-PCR扩增的百合基因组DNA的提取  

Extraction of Genomic DNA for ISSR-PCR Reaction in Lilium

作　　者：陈名红[1,2] 李玉[1] 刘多[1] 熊华斌[1] 李成云[2] 

机构地区：[1]云南民族大学民族药资源化学国家民委-教育部重点实验室,云南昆明650031 [2]云南农业大学农业生物多样性与病虫害控制教育部重点实验室,云南昆明650201

出　　处：《北方园艺》2012年第24期108-110,共3页Northern Horticulture

基　　金：国家自然科学基金资助项目(30860161);云南省教育厅科学研究基金资助项目(2011Y212)

摘　　要：为了方便稳定地获得适合于ISSR-PCR扩增的高质量百合基因组DNA,以卷丹百合为试材,在传统的CTAB法基础上进行了改良和优化,筛选出较理想的DNA提取方法。结果表明:用改良CTAB法提取的百合基因组DNA电泳条带清晰,无降解现象,OD260/OD280值在1.7～2.0之间,用于ISSR-PCR扩增时其稳定性和可重复性都很好,说明DNA纯度和产率完全满足百合ISSR-PCR扩增分析的要求,为百合属植物遗传多样性分析和品种分子鉴别等研究奠定了基础。In order to obtain high quality genornic DNA suitable for ISSR-PCR reaction conveniently and stably in Liliurn, taking Liliurn tigrinrn as matrials, the course of DNA extraction was improved and optimized based on the traditional CTAB method and an ideal method was obtained here. The results showed that the OI;60/O;80 of genomic DNA extracted by the modified CTAB method ranged from 1.7 to 2. 0 with clear electrophoresis strip and its stability and repeatability were very good when used for ISSR-PCR reaction,accounting for its purity and content were all up to the standards for ISSR-PCR analysis. The research laid the foundation for the study on genetic polymorphism analysis, cultivar identification,etc, of Lilium at the molecular level.

关 键 词：百合 基因组DNA DNA提取 ISSR 

分 类 号：S682.29[农业科学—观赏园艺]