苦瓜afp1基因的克隆与序列分析  

Molecular Cloning and Sequence Analysis of afp1 Gene in Momordica charantiap

作　　者：朱美霞[1] 韩改英[1] 王兰明[1] 马兴树[1] 

机构地区：[1]河北工程大学农学院,河北邯郸056038

出　　处：《北方园艺》2012年第24期120-123,共4页Northern Horticulture

基　　金：河北省教育厅科学研究计划资助项目(20091113)

摘　　要：通过异硫氰酸胍法提取苦瓜总RNA,采用一步RT-PCR方法获得394bp苦瓜afp1基因序列。应用DNA star软件进行序列分析,表明这个片段包含了基因完整的编码。Blast分析表明,它与荠菜的防卫基因有很高的同源性,序列一致率为87%。该试验结果将为分子植物抗病育种提供基因材料。Total RNA of Momordica charantiap was extracted using guanidine isothiocyantehod method, afpl gene （394 bp）of Mornordica charantiap was obtained by a step of RT-PCR method, afpl sequence was analyzed using DNA star software, the results showed afpl sequence contained complete open reading frame. Identity percent of it with defensin gene of Brassica juncea was 87%, The results of the project would provide experimental evidence for molecular breeding of plants disease resistance.

关 键 词：苦瓜 afp1 RT-PCR 克隆 序列分析 

分 类 号：Q943.2[生物学—植物学]