农杆菌介导法将高赖氨酸蛋白基因sb401导入玉米的研究  

Transfer of High Lysine Gene sb401 into Maize by Agrobacterium tumefactions Mediated Transformation

作　　者：铁双贵[1,2] 孙静[1,3] 岳润清[1,2] 齐建双[1,2] 王延召[1,2] 柏松[1,3] 陈小洁[1,4] 田保明[3] 

机构地区：[1]河南省农业科学院粮食作物研究所,郑州450002 [2]河南省玉米生物学重点实验室,郑州450002 [3]郑州大学生物工程系,郑州450001 [4]河南师范大学生命科学学院,河南新乡453007

出　　处：《玉米科学》2012年第6期49-53,共5页Journal of Maize Sciences

基　　金：转基因生物新品种培育重大专项

摘　　要：以玉米杂交组合PA×PB的胚性愈伤组织为材料,通过农杆菌介导法将高赖氨酸蛋白基因sb401导入到玉米中。经过双丙胺膦筛选,共获得70株再生植株,其中15株经PCR检测呈阳性,将部分PCR呈阳性的植株进行Southern杂交,结果表明,sb401基因已经整合到玉米基因组中。bar蛋白试纸条检测结果呈阳性,推测目的蛋白得到了表达。High lysine gene sb401 was transferred into embryogenic maize callus of the hybrid line PA x PB by Agrobacterium tumefactions-mediated transformation approach. Fifteen transgenic plants were identified by PCR from 70 regenerated transformed plants obtained by selection for bialaphos resistance. Southern blotting assay for the transgenic plants indicated that sb401 gene has been introduced and integrated into the genome of maize, bar protein dipsticks detection was positive, so we can infer the target protein was expressed.

关 键 词：玉米 农杆菌介导法 高赖氨酸蛋白基因 

分 类 号：S513.035.3[农业科学—作物学]