日本结缕草体细胞变异表型和分子标记的研究  

Phenotypes and molecular markers of somaclonal variation in Zoysia japonica

作　　者：刘莉[1,2] 包满珠[1] 

机构地区：[1]华中农业大学园艺林学学院园艺植物生物学教育部重点实验室,湖北武汉430070 [2]华中农业大学楚天学院,湖北武汉430205

出　　处：《草业科学》2012年第12期1864-1869,共6页Pratacultural Science

基　　金：国家"863"课目资助(2004AA244050)

摘　　要：结缕草(Zoysia japonica)是重要的暖季型草坪草。本研究以1粒种子诱导的愈伤长期继代后的再生植株为材料,在大田中分小区种植,测量其植株表型数据,并同时利用ISSR标记对遗传变异进行分析。在形态和遗传上研究日本结缕草的体细胞变异水平和特征。结果显示,日本结缕草体细胞表型变异中匍匐茎数量和绿色期变异系数较大。10个表型性状经过主成分分析发现,重要表型指标分散,前5个主成分可以代表整体坪用表型,其中第1主成分可以代表直立茎指标,第2主成分代表匍匐茎蔓延能力和生殖枝高度。ISSR标记分析遗传多样性显示,再生植株多态性条带率83.6%,特异性条带15.1%,具有丰富的遗传多样性。本研究可为日本结缕草获得丰富优异变异提供快捷途径,并有利于根据结缕草体细胞变异特点确定育种方向,快速培育新品种。Zoysia japonica is one of important warm-season turfgrasses. The plants as research materials in this study were regenered from calli induced from one seed. Their phenotypes were investigated after planted in field. Furthermore, ISSR was used to detect polymorphism of somaclonal variation. The results showed that variation coefficients of stolon number and green period were bigger than others. Ten pheno- typic characters were analyzed by the Principal Component Analysis, while the first five principal compo- nents could represent whole. The first principal component represented erect stem and the second repre sented stolon and inflorescence branch height. The ISSR marker polymorphic bands and specific bands accounted for 83. 6 % and 15.1% of totalDNAbands, repectively. This research offered a shortcut way for obtaining valuable variations, which made somaclonal variation to contribute to breeding.

关 键 词：ISSR 表型 再生植株 体细胞变异 

分 类 号：S688.4[农业科学—观赏园艺]