甘肃新选育党参品系与主栽品种的RAPD分析  

RAPD analysis of different major cultivated species of Codonopsis pilosula in Gansu

作　　者：杨宁[1] 陈锡莲[1] 高新彦[1] 史万斌 刘效瑞[3] 

机构地区：[1]西北师范大学生命科学学院,甘肃兰州730070 [2]兰州市城关区徐家山林场,甘肃兰州730000 [3]定西市旱作农业科研推广中心,甘肃定西743000

出　　处：《草业科学》2012年第12期1883-1886,共4页Pratacultural Science

基　　金：国家自然科学基金(31160087);甘肃省自然科学基金项目(0803RJZA038);甘肃省教育厅研究生导师项目(1101-06);甘肃省农牧厅项目(GYC09-10);西北师范大学学生科研资助金项目

摘　　要：采用RAPD分子标记技术,分析了新选育党参(Codonopsis pilosula)8个品系和2个主栽品种的遗传多样性和亲缘关系。结果显示,14条引物共扩增出159个位点,其中多态位点26个,多态性位点比率为46.43%,材料间遗传相似系数范围在0.771 9～0.964 9,说明甘肃省主栽党参品种资源具有较丰富的遗传多样性。根据聚类分析结果,党参品种DS6与所有其他供试材料的亲缘关系最远,可单独聚为一类;DS3与DS8的亲缘关系最近,其相似系数为0.964 9。Genetic differences and relationship of 10 （8 lines and 2 cultivars） Codonopsis pilosula species in Gansu were examined by RAPD in this study. The results showed that a total of 159 DNA bands were amplified by 14 oligonucleotide primers, of which 26 bands were polymorphic. The rate of polymorphism was 45.61%. According to the cluster analysis, the 10 C. pilosula species had genetic similarity coefficients ranged from 0. 771 9 to 0. 964 9. The genetic relationship of DS6 species was far from others. However, the genetic relationship between DS3 and DS8 was close with a similarity coefficient of 0. 964 9.

关 键 词：党参 RAPD 遗传多样性 多态位点 

分 类 号：S567.53[农业科学—中草药栽培] Q943[农业科学—作物学]