4种抗原检测日本血吸虫病人血清中特异性IgG和IgG_4考核疗效的比较研究  

COMPARATIVE STUDY ON 4 ANTIGENS FOR EVALUATION OF TREATMENT EFFICACY BY DETECTION OF IgG AND IgG_4 IN SERA OF PATIENTS WITH SCHISTOSOMIASIS JAPONICA

作　　者：张顺科[1] 易新元[1] 舒新华[1] 曾宪芳[1] 曾庆仁[1] 李忠杰[1] 

机构地区：[1]湖南医科大学寄生虫学教研室,长沙410078

出　　处：《中国血吸虫病防治杂志》2000年第3期134-137,共4页Chinese Journal of Schistosomiasis Control

摘　　要：目的探讨ＳｊＭＡｇ、ＫＬＨ及ｒＳｊ３２检测日本血吸虫病人的特异性和敏感性及疗效考核价值。方法采用ＥＬＩＳＡ法，用ＳｊＭＡｇ、ＫＬＨ及ｒＳｊ３２及ＳＥＡ检测治疗前及治疗后不同时期血吸虫病人血清中的特异性ＩｇＧ、ＩｇＧ４及正常人和肺吸虫、肝吸虫、囊虫病人血清中的非特异抗体。结果ＳｊＭＡｇ、ＫＬＨ及ｒＳｊ３２的敏感性和特异性与ＳＥＡ比较差异均无显著性（均Ｐ＞０．０５）；用ＳｊＭＡｇ和ＫＬＨ分别检测治疗后１２个月血清中ＩｇＧ和ＩｇＧ４的阴转率均显著高于ＳＥＡ（Ｐ＜０．０１）、检测治疗后２４个月血清中ＩｇＧ４的阴转率均达９７．６％。结论ＳｊＭＡｇ、ＫＬＨ及ｒＳｊ３２具有与ＳＥＡ相似的诊断价值和较高的疗效考核价值；特异性ＩｇＧ４可能系一种疗效敏感抗体，可望成为疗效考核评价指标。Objective To elucidate the specificity, sensitivity and treatment efficacy of SjMAg, KLH and/or rSj32 in detecting specific IgG and IgG4 antibodies in sera of patients with schistosomiasis japonica before and after treatment. Methods SjMAg, KLH and/or rSj32 were used in ELISA to determine the specific IgG and IgG4 in sera of patients with schistosomiasis japonica before and after treatment, and the cross-reactivity was tested with sera of normal persons and patients with paragonimiasis or clonorchiasis or cysticercosis. Results SjMAg, KLH and rSj32 were as sensitive and specific as SEA in diagnosis of schistosomiasis japonica. The respective negative conversion rates of IgG and IgG, detected by SjMAg, KLH and rSj32 12 months after treatment were significantly higher than that by SEA. The negative conversion rates of IgG. detected by either SjMAg or KLH 24 months after treatment were similar as 97.6%. Conclusion SjMAg, KLH and rSj32 showed the same value as SEA in diagnosing schistosomiasis japonica and higher value in assessing therapeutic efficacy. The specific IgG4 may be a short-duration antibody which can indicate successful chemotherapy of patients with Schistosoma japonicun infection.

关 键 词：日本血吸虫病 血清诊断 IGG IGG4 抗原 

分 类 号：R532.210.4[医药卫生—内科学]