农杆菌介导蔗糖：蔗糖果糖基转移酶基因转化木薯的研究  

Agrobacterium-Mediated Transformation of Cassava for Sucrose: Sucrose1-Fructosyltransferase Gene

作　　者：王颖[1] 郭军辉[1] 陈雄庭[1] 王文泉[1] 吴坤鑫[1] 王海燕[1] 

机构地区：[1]中国热带农业科学院热带生物技术研究所农业部热带作物生物学与遗传资源利用重点实验室,海南海口571101

出　　处：《热带作物学报》2012年第11期2002-2006,共5页Chinese Journal of Tropical Crops

基　　金：中南美洲热带作物种质资源收集与合作研究(No.2011DFB31690);中央级公益性科研院所基本科研业务费专项资金资助项目(No.ITBB110203)

摘　　要：蔗糖:蔗糖果糖基转移酶1-SST是果聚糖合成代谢过程的关键酶,而果聚糖的积累可以增强植物在多种环境胁迫下的抗逆性。通过农杆菌介导法将1-SST基因导入木薯品种SC6中。结果表明:芽器官发生时PPT的最佳筛选浓度为2.0 mg/L,共得到71株抗性再生植株。对抗性植株进行了PCR、RT-PCR鉴定,其中阳性植株2株,经Southern杂交分析,最终获得1株转基因木薯植株。Sucrose：sucrose 1-fructosyhransferase （1-SST）is one of the key enzymes which mainly involves in biosynthesis of fructan, the resistance of plant in a variety of environmental stress is enhanced with fructanaccumulation. In this study, 1-SST gene was successful introduced into cassava SC6 genome by Agrobacterium-mediated transformation. The results showed that, the best screening concentration of PPT was 2.0 mg/L while budding, 71 resistant plants were obtained, two positives among them were found by PCR and RT- PCR analysis. Southern blot analysis showed that 1-SST gene was integrated into the genome of one transgenic cassava plant.

关 键 词：农杆菌介导 1-SST基因 木薯 

分 类 号：Q812[生物学—生物工程]