宫清方诱导Th1/Th2亚群漂移减轻药物流产后子宫出血动物研究  

Gongqing decoction reduces the volume of uterine bleeding in RU486 medical abortion by inducing the shift of Th1/Th2 subsets

作　　者：李霞[1] 王永红 王丽[1] 赵向忠[1] 周宪宾[1] 郭钰琪[1] 姜国胜[1] 姚成芳[1] 

机构地区：[1]山东省医学科学院基础医学研究所,济南250062 [2]山东省肥城市中医院妇产科

出　　处：《中国计划生育学杂志》2012年第12期805-808,共4页Chinese Journal of Family Planning

基　　金：国家自然科学基金项目(30901918);山东省科技发展计划项目(2012GSF11840);山东省博士基金项目(BS2009YY041);山东省自然科学基金项目(ZR2010HQ066);山东省人口计生委项目([2009]8);山东省中管局项目(2009-173)

摘　　要：目的:探讨药物流产中Th1/Th2亚群漂移与子宫出血量的相关性以及宫清方减轻药物流产后子宫出血的作用机制。方法:将孕鼠20只随机分为米非司酮大(1.5mg/kg·d)、小(0.88mg/kg·d)剂量组各10只,改良碱性正铁血红素法检测子宫出血量,流式细胞术双染色法检测母胎界面Th1、Th2亚群比例;孕鼠40只随机分为米非司酮组(1.5mg/kgod)、宫清方大、中、小剂量组各10只,检测子宫出血量及母胎界面Th1、Th2亚群比例;逆转录-聚合酶链式反应检测母胎界面干扰素-γ(IFN-γ)、白介素-4(IL-4)mRNA转录水平。结果:米非司酮大剂量组小鼠子宫出血量<米非司酮小剂量组(P<0.05),药物流产小鼠子宫出血量与Th1亚群比例、Th1/Th2比值呈负相关(P<0.05),与Th2亚群比例呈正相关(P<0.05)。宫清方能明显减少米非司酮药物流产小鼠子宫出血量(P<0.05),升高IFN-γmRNA表达及Th1亚群比例(P<0.05),降低IL-4mRNA表达及Th2亚群比例(P<0.05),升高Th1/Th2比值(P<0.05)。结论:米非司酮药物流产中Th1/Th2亚群漂移与子宫出血量密切相关,宫清方通过优势诱导药物流产小鼠母胎界面Th1/Th2平衡向Th1漂移,减轻子宫出血。Objective ： To investigate the correlation between Th1/Th2 shift and the volume of uterine bleeding in RU486 med- ical abortion, and to study the mechanism of ＂Gongqing decoction＂ in reducing the volume of uterine bleeding in RU486 med- ical abortion. Methods： 20 pregnant mice were assigned into RU486 high- dose （1.5 mg/kgod） and low- dose （0.88 mg/ kgod） medical abortion groups randomly. The volume of uterine bleeding was measured by the method of alkaline hematin photometry. The percentages of Thl subset and Th2 subset in the maternofetal interface were determined by flow cytometry. Subsequently, 40 RU486 pregnant mice were assigned into RU486 group （ 1.5 mg/kgod）, Gongqing decoction high - dose （308.16 mg/kgod）, mid-dose （154.08 mg/kgod） and low- dose （77.04 mg/kgod） groups randomly. Uterine bleeding volume and Th1/Th2 subsets were assessed by the same methods above. In addition, the mRNA expressions of IFN - γ and IL - 4 were assessed by RT - PCR. Results ： The uterine bleeding volume in RU486 high - dose group was much lower than that in RU486 low - dose group （ P 〈 0.05 ）, and the uterine bleeding volume was negatively correlated with the pereentage of Thl subset and the ratio of Thl/Th2 （ P 〈 0.05 ）, while positively correlated with the percentage of Th2 subset （ P 〈 0.05 ）. Gongqing decoction reduced the uterine bleeding volume efficiently in RU486 medical abortion （ P 〈 O. 05 ）. The mRNA expression of IFN - γ and the percentage of Thl cells were up - regulated while the mRNA expression of IL - 4 and the per- centage of Th2 cells were down - regulated after Gongqing decoction administration （ P 〈 0. 05 ）. Conclusion ： The uterine bleeding volume was closely related to Thl/Th2 paradigm in RU486 medical abortion. Gongqing decoction reduced uterine bleeding volume efficiently by inducing Thl skew.

关 键 词：宫清方 药物流产 子宫出血 TH1 TH2 

分 类 号：R285.5[医药卫生—中药学]